ADAMTS10 Monoclonal Antibody | anti-ADAMTS10 antibody

ADAMTS10 (a Metallopeptidase with thrombospondin type 1 motif, 10)

Cat. Num. AAA605702

• Gene Names: ADAMTS10; WMS; ADAM-TS10
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Purified by Protein G affinity chromatography from hybridoma culture supernatent.
• Synonyms: ADAMTS10; Monoclonal Antibody; ADAMTS10 (a Metallopeptidase with thrombospondin type 1 motif; 10); Anti -ADAMTS10 (a Metallopeptidase with thrombospondin type 1 motif; anti-ADAMTS10 antibody

• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9H44
• Specificity: Recognizes human ADAMTS10. In direct ELISA, does not crossreact with recombinant human ADAMTS1 3,4,5,12,13,15,16,or L2 is observed.
• Purity/Purification: Purified by Protein G affinity chromatography from hybridoma culture supernatent.
• Form/Format: Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

• Applicable Applications for anti-ADAMTS10 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Western Blot: 1ug/ml of recombinant ADAMTS10 under non-reducing conditions only; Immunoprecipitation: 25ug/ml from conditioned cell culture medium spiked with recombinant human ADAMTS10; Optimal dilutions to be determined by the researcher.
• Immunogen: Recombinant protein corresponding to Phe26-Gly1102 from human ADAMTS10, expressed in CHO cells.
• Type: Mab
• Grade: Affinity Purified
• Preparation and Storage: Lyophilized and reconstituted products are stable for 12months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ADAMTS10 antibody

ADAMTS10 belongs to the ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin type-1 motifs) family of zinc-dependent metalloproteases. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. Mutations in the ADAMTS10 gene may cause autosomal recessive Weill-Marchesani syndrome. The amino acid sequence of human ADAMTS10 is 100%, 96% and 94% identical to that of chimpanzee, dog and mouse.

Product Categories/Family for anti-ADAMTS10 antibody

Antibodies; Abs to Matrix Metalloproteinases

NCBI and Uniprot Product Information

• NCBI GI #: 56121815
• NCBI GeneID: 81794
• NCBI Accession #: NP_112219.2
• NCBI GenBank Nucleotide #: NM_030957.2
• UniProt Accession #: Q9H324; Q59FE5; Q6ZN14
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 10 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 10
• NCBI Official Symbol: ADAMTS10
• NCBI Official Synonym Symbols: WMS; ADAM-TS10
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 10; ADAMTS-10; ADAM-TS 10; zinc metalloendopeptidase; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 10
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 10
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS10
• UniProt Entry Name: ATS10_HUMAN

NCBI Description

This gene belongs to the ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin type-1 motifs) family of zinc-dependent proteases. ADAMTS proteases are complex secreted enzymes containing a prometalloprotease domain of the reprolysin type attached to an ancillary domain with a highly conserved structure that includes at least one thrombospondin type 1 repeat. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. The product of this gene plays a major role in growth and in skin, lens, and heart development. It is also a candidate gene for autosomal recessive Weill-Marchesani syndrome. [provided by RefSeq]

Uniprot Description

ADAMTS10: Metalloprotease that participate in microfibrils assembly. Microfibrils are extracellular matrix components occurring independently or along with elastin in the formation of elastic tissues. Defects in ADAMTS10 are the cause of Weill-Marchesani syndrome 1 (WMS1). WMS1 is a rare connective tissue disorder characterized by short stature, brachydactyly, joint stiffness, and eye abnormalities including microspherophakia, ectopia lentis, severe myopia and glaucoma.

Protein type: Secreted; EC 3.4.24.-; Protease; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular matrix; microfibril

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity

Biological Process: proteolysis

Disease: Weill-marchesani Syndrome 1