Western Blot (WB)
(Western blot analysis of AID in Ramos|whole cell lysate with AID antibody at 2ug/ml in either the (A) absence or (B)|presence of blocking peptide.)
Activation-induced Cytidine Deaminase Enzyme | AICDA enzyme
Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, HIGM2) (Control Peptide)
Highly Purified
Highly Purified
Dilution: Western Blot: AID peptide is used for blocking the activity of AID antibody (Cat. No. A0855-75J). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37 C.
Western Blot (WB)
(Western blot analysis of AID in Ramos|whole cell lysate with AID antibody at 2ug/ml in either the (A) absence or (B)|presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of AID in Ramos|whole cell lysate with AID antibody at 2ug/ml in either the (A) absence or (B)|presence of blocking peptide.)
Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNAediting cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines (1). It is necessary for somatic hypermutation and class switch recombination in B cells (2), but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms (2,3). Although it is structurally and functionally similar to the APOBEC proteins (reviewed in 4), it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G (5).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Feb 2009]
Uniprot Description
Function: Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Ref.9 Ref.10 Ref.11 Ref.13
Catalytic activity: Cytosine in single-stranded DNA + H2O = uracil in single-stranded DNA + NH3.
Cofactor: Zinc
By similarity.
Subunit structure: Interacts with CTNNBL1; the interaction is important for the immunoglobulin switch activity of AICDA. Interacts (via its NLS) with KPNA1. Interacts with PKA/PRKACA and PRKAR1A/PKR1. Interacts with TRIM28 and NCL
By similarity. Interacts with SUPT6H. Ref.8 Ref.9 Ref.11 Ref.13
Subcellular location: Nucleus. Cytoplasm. Note: Predominantly cytoplasmic but shuttles between the nucleus and the cytoplasm. Ref.7 Ref.9 Ref.11 Ref.12
Tissue specificity: Strongly expressed in lymph nodes and tonsils.
Post-translational modification: Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity.
Involvement in disease: Immunodeficiency with hyper-IgM 2 (HIGM2) [MIM:605258]: A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE. It results in a profound susceptibility to bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2
Sequence similarities: Belongs to the cytidine and deoxycytidylate deaminase family.
Research Articles on AICDA
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Product Notes
The AICDA aicda (Catalog #AAA653625) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. Suitable for use as a blocking peptide in ELISA, Western Blot with A0855-75J. Dilution: Western Blot: AID peptide is used for blocking the activity of AID antibody (Cat. No. A0855-75J). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37 C. Researchers should empirically determine the suitability of the AICDA aicda for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Activation-induced Cytidine Deaminase, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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