ACP2 recombinant protein

ACP2, 31-380aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA206114

• Gene Names: ACP2; LAP
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: ACP2; 31-380aa; Human; His tag; E Coli (Denatured); Lysosomal acid phosphatase isoform 1; LAP; ACP2 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid, In 20mM Tris-HCl (pH 8.0) containing 10% glycerol
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSRSLRFVT LLYRHGDRSP VKTYPKDPYQ EEEWPQGFGQ LTKEGMLQHW ELGQALRQRY HGFLNTSYHR QEVYVRSTDF DRTLMSAEAN LAGLFPPNGM QRFNPNISWQ PIPVHTVPIT EDRLLKFPLG PCPRYEQLQN ETRQTPEYQN ESSRNAQFLD MVANETGLTD LTLETVWNVY DTLFCEQTHG LRLPPWASPQ TMQRLSRLKD FSFRFLFGIY QQAEKARLQG GVLLAQIRKN LTLMATTSQL PKLLVYSAHD TTLVALQMAL DVYNGEQAPY ASCHIFELYQ EDSGNFSVEM YFRNESDKAP WPLSLPGCPH RCPLQDFLRL TEPVVPKDWQ QECQLASGPA DTE
• Sequence Length: 423

• Applicable Applications for ACP2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ACP2 recombinant protein

ACP2 also known as Lysosomal acid phosphatase isoform 1. ACP2 is composed of two subunits, alpha and beta, and is chemically and genetically distinct from red cell acid phosphatase. it is a member of a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. Acid phosphatase deficiency is caused by mutations in the ACP2 (beta subunit) and ACP3 (alpha subunit) genes. Recombinant human ACP2, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for ACP2 recombinant protein

Enzymes & Proteases

References

Moss DW., et al. (1995) Critical reviews in clinical laboratory sciences, 32 (4): 431-67

NCBI and Uniprot Product Information

• NCBI GI #: 4557010
• NCBI GeneID: 53
• NCBI Accession #: NP_001601
• NCBI GenBank Nucleotide #: NM_001610.3
• UniProt Accession #: P11117; Q561W5; Q9BTU7; E9PCI1
• Molecular Weight: 42.9kDa (373aa)
• NCBI Official Full Name: lysosomal acid phosphatase isoform 1
• NCBI Official Synonym Full Names: acid phosphatase 2, lysosomal
• NCBI Official Symbol: ACP2
• NCBI Official Synonym Symbols: LAP
• NCBI Protein Information: lysosomal acid phosphatase
• UniProt Protein Name: Lysosomal acid phosphatase
• Protein Family: Cysteine proteinase
• UniProt Gene Name: ACP2
• UniProt Synonym Gene Names: LAP

NCBI Description

The protein encoded by this gene belongs to the histidine acid phosphatase family, which hydrolyze orthophosphoric monoesters to alcohol and phosphate. This protein is localized to the lysosomal membrane, and is chemically and genetically distinct from the red cell acid phosphatase. Mice lacking this gene showed multiple defects, including bone structure alterations, lysosomal storage defects, and an increased tendency towards seizures. An enzymatically-inactive allele of this gene in mice showed severe growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype. Alternatively spliced transcript variants have been found for this gene. A C-terminally extended isoform is also predicted to be produced by the use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism. [provided by RefSeq, Oct 2017]