Mouse anti-Human ACE2 (SARS Receptor) Monoclonal Antibody | anti-ACE2 antibody

ACE2 (SARS Receptor) Antibody (Center) (Ascites)

Cat. Num. AAA9200360

• Gene Names: ACE2; ACEH
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: ACE2 (SARS Receptor); Monoclonal Antibody; ACE2 (SARS Receptor) Antibody (Center) (Ascites); Angiotensin-converting enzyme 2; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; ACEH; Metalloprotease MPROT15; Processed angiotensin-converting enzyme 2; ACE2; anti-ACE2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM
• Specificity: Purified His-tagged ACE2 protein was used to produced this monoclonal antibody.
• Form/Format: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
• Sequence Positions: 1-270
• Sequence Length: 805

• Applicable Applications for anti-ACE2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Function: Essential counter-regulatory carboxypeptidase of the renin- angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed: 27217402). Converts angiotensin I to angiotensin 1- 9, anine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed: 10969042, PubMed: 10924499, PubMed: 11815627,PubMed: 19021774,PubMed:14504186). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed: 10969042,PubMed: 11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13,apelin-17, apelin-36), casomorphins (beta-casomorphin- 7, neocasomorphin) and dynorphin A with high efficiency (PubMed: 11815627,PubMed: 27217402, PubMed: 28293165). Inaddition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed: 18424768, PubMed: 19185582).
• Cellular Location: [Processed angiotensin-converting enzyme 2]: Secreted [Isoform 2]: Apical cell membrane
• Tissue Location: Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells (at protein level) (PubMed:15141377). Expressed in enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level) (PubMed:15141377) Expressed in the renal proximal tubule and the small intestine (at protein level) (PubMed:18424768). Expressed in heart, kidney, testis, and gastrointestinal system (at protein level) (PubMed: 10969042, PubMed: 10924499, PubMed: 15231706, PubMed: 12459472, PubMed: 15671045, PubMed: 32715618, PubMed: 32170560). In lung, expressed at low levels in some alveolar type 2 cells, the expression seems to be individual- specific (at protein level) (PubMed: 32425701, PubMed: 15141377, PubMed: 32715618, PubMed: 32170560, PubMed: 33432184). Expressed in nasal epithelial cells (at protein level) (PubMed: 33432184). Coexpressed with TMPRSS2 within some lung alveolar type 2 cells, ileal absorptive enterocytes, intestinal epithelial cells, cornea, gallbladder and nasal goblet secretory cells (PubMed: 32413319, PubMed: 32327758, PubMed: 32358202). Coexpressed with TMPRSS4 within mature enterocytes (PubMed:32404436).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks . For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(ACE2 (SARS Receptor) Antibody (Center)(MBS9200360) western blot analysis in MDA-MB-453,MCF-7 cell line lysates (35ug/lane).This demonstrates the ACE2 (SARS Receptor) antibody detected the ACE2 (SARS Receptor) protein (arrow).)

Related Product Information for anti-ACE2 antibody

Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.

Product Categories/Family for anti-ACE2 antibody

Crown Antibodies; Cardiovascular; Cell Biology

References

Donoghue M., et al. Circ. Res. 87:E1-E9(2000).
Tipnis S.R., et al. J. Biol. Chem. 275:33238-33243(2000).
Douglas G.C., et al. Endocrinology 145:4703-4711(2004).
Itoyama S., et al. Am. J. Med. Genet. A 136:52-57(2005).
Suzuki Y., et al. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 11225609
• NCBI GeneID: 59272
• NCBI Accession #: NP_068576.1
• NCBI GenBank Nucleotide #: NM_021804.2
• UniProt Accession #: Q9BYF1; Q6UWP0; Q86WT0; Q9NRA7; Q9UFZ6; C7ECU1
• NCBI Official Full Name: angiotensin-converting enzyme 2
• NCBI Official Synonym Full Names: angiotensin I converting enzyme 2
• NCBI Official Symbol: ACE2
• NCBI Official Synonym Symbols: ACEH
• NCBI Protein Information: angiotensin-converting enzyme 2
• UniProt Protein Name: Angiotensin-converting enzyme 2
• Protein Family: Angiotensin-converting enzyme
• UniProt Gene Name: ACE2
• UniProt Synonym Gene Names: ACEH
• UniProt Entry Name: ACE2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

Uniprot Description

ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protease; EC 3.4.17.23

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: extracellular space; cell surface; cytoplasm; plasma membrane; integral to membrane; extracellular region; lipid raft

Molecular Function: peptidyl-dipeptidase activity; viral receptor activity; protein binding; metallopeptidase activity; zinc ion binding; carboxypeptidase activity; peptide hormone binding; endopeptidase activity; glycoprotein binding

Biological Process: virion attachment, binding of host cell surface receptor; entry of virus into host cell; maternal process involved in pregnancy; regulation of vasodilation; angiotensin maturation; proteolysis; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of cell proliferation; regulation of cytokine production; cellular protein metabolic process; regulation of vasoconstriction; regulation of inflammatory response; angiotensin catabolic process in blood; receptor biosynthetic process; angiotensin mediated drinking behavior