PTPN12 active protein

PTPN12 Protein, Human, Recombinant (aa 1-355, His & GST Tag)

Cat. Num. AAA8120852

• Gene Names: PTPN12; PTPG1; PTP-PEST
• Purity: >90% as determined by SDS-PAGE
• Synonyms: PTPN12; PTPN12 Protein; Human; Recombinant (aa 1-355; His & GST Tag); Human PTPN12 Protein (aa 1-355; PTP-PEST Protein; PTPG1 Protein; protein tyrosine phosphatase; non-receptor type 12; PTPN12 active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln355

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human PTPN12 (AAA36529.1)(Met1-Gln355) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate The specific activity is >15uMoles/min/mg.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PTPN12 active protein

Background: PTPN12 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN12 contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may be related to protein intracellular half-life. PTPN12 was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. PTPN12 was shown to interact with, and dephosphorylate, various cytoskeleton and cell adhesion molecules, such as p13 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobility.

References

Garton AJ. et al., 1997, Oncogene. 15 (8): 877-85. Lin Yi. et al., 2003, Am J Physiol Heart Circ. 285 (2): H710-21. Takekawa M. et al., 1994, FEBS Lett. 339 (3): 222-8.

NCBI and Uniprot Product Information

• NCBI GI #: 292409
• NCBI GeneID: 5782
• Molecular Weight: 74,138 Da
• NCBI Official Full Name: protein tyrosine phosphatase
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 12
• NCBI Official Symbol: PTPN12
• NCBI Official Synonym Symbols: PTPG1; PTP-PEST
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 12
• Protein Family: Tyrosine-protein phosphatase non-receptor

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may regulate protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of the oncogene c-ABL and thus may play a role in oncogenesis. This PTP was also shown to interact with, and dephosphorylate, various products related to cytoskeletal structure and cell adhesion, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin. This suggests it has a regulatory role in controlling cell shape and mobility. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]