Rabbit RIPK2 Polyclonal Antibody | anti-RIPK2 antibody

RIPK2 Antibody

Cat. Num. AAA9404707

• Gene Names: RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: RIPK2; Polyclonal Antibody; RIPK2 Antibody; CCK; RICK; RIP2; CARD3; GIG30; anti-RIPK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total RIPK2 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 540

• Applicable Applications for anti-RIPK2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human RIPK2.
• Target Name: RIPK2
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using RIPK2 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver cancer using RIPK2 antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse kidney using RIPK2 antibody at dilution of 1:100 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using RIPK2 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-RIPK2 antibody

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli.

Product Categories/Family for anti-RIPK2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4506537
• NCBI GeneID: 8767
• NCBI Accession #: NP_003812.1
• NCBI GenBank Nucleotide #: NM_003821.5
• UniProt Accession #: O43353; Q6UWF0; B7Z748
• Molecular Weight: 45,582 Da
• NCBI Official Full Name: receptor-interacting serine/threonine-protein kinase 2
• NCBI Official Synonym Full Names: receptor-interacting serine-threonine kinase 2
• NCBI Official Symbol: RIPK2
• NCBI Official Synonym Symbols: CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 2
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 2
• Protein Family: Receptor-interacting serine/threonine-protein kinase
• UniProt Gene Name: RIPK2
• UniProt Synonym Gene Names: CARDIAK; RICK; RIP2; CARD-containing IL-1 beta ICE-kinase; RIP-2
• UniProt Entry Name: RIPK2_HUMAN

NCBI Description

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq, Jul 2008]

Uniprot Description

RIPK2: a tyrosine kinase-like kinase of the RIPK family. Activates pro-caspase-1 and pro-caspase-8. Potentiates casp-8-mediated apoptosis. May activate NF-kappaB.

Protein type: EC 2.7.11.1; EC 2.7.10.2; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; RIPK family

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: cytoskeleton; protein complex; cytoplasm; cytosol; vesicle

Molecular Function: protein serine/threonine kinase activity; signal transducer activity; protein binding; LIM domain binding; protein homodimerization activity; non-membrane spanning protein tyrosine kinase activity; CARD domain binding; ATP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; activation of MAPK activity; positive regulation of interleukin-12 production; apoptosis; positive regulation of apoptosis; stress-activated MAPK cascade; positive regulation of JNK cascade; toll-like receptor 3 signaling pathway; signal transduction; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; T cell proliferation; toll-like receptor 5 signaling pathway; response to exogenous dsRNA; positive regulation of immature T cell proliferation; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon-beta production; JNK cascade; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; adaptive immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of peptidyl-serine phosphorylation; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of interferon-gamma production; defense response to Gram-positive bacterium; positive regulation of protein ubiquitination; positive regulation of interleukin-2 production; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of alpha-beta T cell proliferation; toll-like receptor 9 signaling pathway; positive regulation of cytokine and chemokine mediated signaling pathway; positive regulation of T-helper 1 cell differentiation; negative regulation of apoptosis