Rat Insulin-like growth factor-binding protein 6 ELISA Kit | IGFBP-6 elisa kit

Rat Insulin-like growth factor-binding protein 6 ELISA Kit

Cat. Num. AAA761445

• Gene Names: IGFBP6; IBP6
• Reactivity: Rat
• Synonyms: Insulin-like growth factor-binding protein 6; Rat Insulin-like growth factor-binding protein 6 ELISA Kit; IGFBP-6/IGFBP6/IBP6; IGFBP-6 elisa kit

• Reactivity: Rat
• Sequence Length: 240

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for IGFBP-6 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat IGFBP-6 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat IGFBP-6 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat IGFBP-6 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat IGFBP-6 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 11321593
• NCBI GeneID: 3489
• NCBI Accession #: NP_002169.1
• NCBI GenBank Nucleotide #: NM_002178.2
• Molecular Weight: 25,322 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 6
• NCBI Official Synonym Full Names: insulin like growth factor binding protein 6
• NCBI Official Symbol: IGFBP6
• NCBI Official Synonym Symbols: IBP6
• NCBI Protein Information: insulin-like growth factor-binding protein 6
• UniProt Protein Name: Insulin-like growth factor-binding protein 6
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP6
• UniProt Synonym Gene Names: IBP6; IBP-6; IGF-binding protein 6; IGFBP-6
• UniProt Entry Name: IBP6_HUMAN

Uniprot Description

IGFBP6: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Secreted; Cell cycle regulation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular region; extracellular space; Golgi apparatus

Molecular Function: insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: cellular protein metabolic process; negative regulation of cell proliferation; regulation of cell growth; regulation of insulin-like growth factor receptor signaling pathway; signal transduction