Western Blot/Blotting
(Western blot analysis using Luciferase antibody on recombinant luciferase protein.)
Mouse Luciferase Monoclonal Antibody
Luciferase (N-Terminus)
Reactivity
Luciferase (Firefly) Protein
Applications
Western Blot, Immunohistochemistry
Purity
Protein A/G Chromatography
Synonyms
Luciferase; Monoclonal Antibody; Luciferase (N-Terminus); anti-Luciferase antibody
Host
Mouse
Reactivity
Luciferase (Firefly) Protein
Clonality
Monoclonal
Isotype
IgG1
Clone Number
Luci17
Purity/Purification
Protein A/G Chromatography
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Applicable Applications for anti-Luciferase antibody
Western Blot (WB), Immunohistochemistry (IHC)
Immunogen
Hybridoma produced by the fusion of splenocytes from mice immunized with luciferase protein isolated from Photinus pyralis.
Positive Control
Purified luciferase protein
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
Western Blot/Blotting
(Western blot analysis using Luciferase antibody on recombinant luciferase protein.)
Western Blot/Blotting
(Western blot analysis using Luciferase antibody on recombinant luciferase protein.)
Related Product Information for anti-Luciferase antibody
Analysis of gene expression is most commonly assayed by transient transfection. Systems are generally based on the use of fusion genes which are inserted into cells, and the gene expression is assayed within 48 hours after introduction of DNA. Usually the fusion consists of the promoter binding site or enhancer sequence under study which is attached to a reporter gene. The amount of the reporter protein synthesized under the experimental conditions, is presumed to reflect the ability of the sequences studied to direct or promote transcription. Several enzymes are commonly used as reporter proteins, among them are chloramphenicol acetyl transferase (CAT), -galactosidase, human growth hormone (hGH) and luciferase. Luciferase has become one of the widely used reporter enzymes. The enzyme catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg+2 and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. The use of an antibody to detect luciferase can provide an alternative detection assay which directly detects luciferase protein levels, and thus has the advantage that it does not require luciferase activity and is not dependent on rapid kinetics. Moreover, antibodies can detect the luciferase enzyme expression in situ, providing a means to study the localized signal sequences using luciferase as a reporter gene.
Product Categories/Family for anti-Luciferase antibody
Similar Products
Product Notes
The Luciferase (Catalog #AAA395251) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Luciferase (N-Terminus) reacts with Luciferase (Firefly) Protein and may cross-react with other species as described in the data sheet. AAA Biotech's Luciferase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the Luciferase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Luciferase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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