Western Blot (WB)
(Western blot analysis of 14-3-3 Theta on different lysates using anti-14-3-3 Theta antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7)
Rabbit 14-3-3 Theta Monoclonal Antibody | anti-YWHAQ antibody
14-3-3 Theta Antibody
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
Western Blot (WB)
(Western blot analysis of 14-3-3 Theta on different lysates using anti-14-3-3 Theta antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7)
Western Blot (WB)
(Western blot analysis of 14-3-3 Theta on different lysates using anti-14-3-3 Theta antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7)
Immunocytochemistry (ICC)
(ICC staining 14-3-3 Theta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining 14-3-3 Theta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining 14-3-3 Theta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining 14-3-3 Theta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining 14-3-3 Theta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining 14-3-3 Theta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 Theta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 Theta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]
Uniprot Description
14-3-3 theta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Associates with c-BCR and BCR-Abl.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 2p25.1
Cellular Component: cytoplasmic vesicle membrane; focal adhesion; membrane; cytoplasm; cytosol
Molecular Function: protein domain specific binding; protein binding; protein N-terminus binding
Biological Process: substantia nigra development; apoptosis; small GTPase mediated signal transduction; negative regulation of transcription, DNA-dependent; protein targeting
Research Articles on YWHAQ
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Product Notes
The YWHAQ ywhaq (Catalog #AAA9435973) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The 14-3-3 Theta Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 Theta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:1000-5000 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the YWHAQ ywhaq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 Theta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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