Human 14-3-3 protein theta ELISA Kit | YWHAQ elisa kit

Human 14-3-3 protein theta ELISA Kit

Cat. Num. AAA763827

• Gene Names: YWHAQ; 1C5; HS1; 14-3-3
• Reactivity: Human
• Synonyms: 14-3-3 protein theta; Human 14-3-3 protein theta ELISA Kit; YWHAQ/14-3-3 protein theta/Protein HS1/14-3-3 protein T-cell/14-3-3 protein tau; YWHAQ elisa kit

• Reactivity: Human
• Sequence Length: 245

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for YWHAQ elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human YWHAQ antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human YWHAQ antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human YWHAQ amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human YWHAQ can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 5803227
• NCBI GeneID: 10971
• NCBI Accession #: NP_006817.1
• NCBI GenBank Nucleotide #: NM_006826.3
• Molecular Weight: 27,764 Da
• NCBI Official Full Name: 14-3-3 protein theta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta
• NCBI Official Symbol: YWHAQ
• NCBI Official Synonym Symbols: 1C5; HS1; 14-3-3
• NCBI Protein Information: 14-3-3 protein theta
• UniProt Protein Name: 14-3-3 protein theta
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAQ
• UniProt Entry Name: 1433T_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 theta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Associates with c-BCR and BCR-Abl.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; focal adhesion; membrane; mitochondrion; protein complex

Molecular Function: protein binding; protein C-terminus binding; protein domain specific binding; protein N-terminus binding

Biological Process: apoptosis; gene expression; negative regulation of transcription, DNA-dependent; programmed cell death; small GTPase mediated signal transduction; substantia nigra development; transcription initiation from RNA polymerase II promoter