Porcine c-myc Oncogene product ELISA Kit | C-MYC elisa kit

Porcine c-myc Oncogene product ELISA Kit

Cat. Num. AAA737188

• Gene Names: MYC; MRTL; MYCC; c-Myc; bHLHe39
• Reactivity: Porcine
• Synonyms: c-myc Oncogene product; Porcine c-myc Oncogene product ELISA Kit; C-MYC elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This Cmyc ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine Cmyc. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C-MYC elisa kit

Principle of the Assay: C-MYC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C-MYC antibody and an C-MYC-HRP conjugate. The assay sample and buffer are incubated together with C-MYC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C-MYC concentration since C-MYC from samples and C-MYC-HRP conjugate compete for the anti-C-MYC antibody binding site. Since the number of sites is limited, as more sites are occupied by C-MYC from the sample, fewer sites are left to bind C-MYC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C-MYC concentration in each sample is interpolated from this standard curve.

Product Categories/Family for C-MYC elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 396512
• NCBI GeneID: 4609
• UniProt Accession #: Q6LBK7; P01106
• Molecular Weight: 7,012 Da
• NCBI Official Full Name: c-myc, partial
• NCBI Official Synonym Full Names: v-myc avian myelocytomatosis viral oncogene homolog
• NCBI Official Symbol: MYC
• NCBI Official Synonym Symbols: MRTL; MYCC; c-Myc; bHLHe39
• NCBI Protein Information: myc proto-oncogene protein; proto-oncogene c-Myc; transcription factor p64; class E basic helix-loop-helix protein 39; avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog; myc-related translation/localization regulatory factor
• UniProt Protein Name: C-myc protein
• UniProt Gene Name: c-myc
• UniProt Entry Name: Q6LBK7_HUMAN

NCBI Description

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Myc: a proto-oncogenic transcription factor that plays a role in cell proliferation, apoptosis and in the development of human tumors. Seems to activate the transcription of growth-related genes.

Protein type: DNA-binding; Nucleolus; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 8q24.21

Cellular Component: nucleoplasm; protein complex; nucleolus; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; DNA binding; protein complex binding; transcription factor binding; transcription factor activity

Biological Process: cellular iron ion homeostasis; oxygen transport; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; chromosome organization and biogenesis; positive regulation of fibroblast proliferation; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to gamma radiation; cell cycle arrest; response to drug; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; regulation of telomere maintenance; MAPKKK cascade; negative regulation of cell division; negative regulation of monocyte differentiation; negative regulation of stress-activated MAPK cascade; chromatin remodeling; negative regulation of fibroblast proliferation; ureteric bud branching; regulation of gene expression; energy reserve metabolic process; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus; positive regulation of epithelial cell proliferation; negative regulation of apoptosis

Disease: Burkitt Lymphoma