Monkey MAP-2 ELISA Kit | MAP-2 elisa kit

Monkey MAP-2 (Microtubule Associated Protein 2) ELISA Kit

Cat. Num. AAA2510596

• Gene Names: MAP2; MAP2A; MAP2B; MAP2C
• Reactivity: Monkey
• Synonyms: MAP-2; Monkey MAP-2 (Microtubule Associated Protein 2) ELISA Kit; MAP-2 elisa kit

• Reactivity: Monkey
• Specificity: This kit recognizes natural and recombinant Monkey MAP-2. No significant cross-reactivity or interference between Monkey MAP-2 and analogues was observed.
• Sequence Length: 1827

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Related Product Information for MAP-2 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey MAP-2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MAP-2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MAP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MAP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MAP-2. You can calculate the concentration of MAP-2 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 87578396
• NCBI GeneID: 4133
• NCBI Accession #: NP_002365.3
• NCBI GenBank Nucleotide #: NM_002374.3
• UniProt Accession #: P11137; Q17S04; Q8IUX2; Q99975; Q99976
• Molecular Weight: 58,954 Da
• NCBI Official Full Name: microtubule-associated protein 2 isoform 1
• NCBI Official Synonym Full Names: microtubule-associated protein 2
• NCBI Official Symbol: MAP2
• NCBI Official Synonym Symbols: MAP2A; MAP2B; MAP2C
• NCBI Protein Information: microtubule-associated protein 2; MAP-2
• UniProt Protein Name: Microtubule-associated protein 2
• UniProt Gene Name: MAP2
• UniProt Synonym Gene Names: MAP-2
• UniProt Entry Name: MTAP2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]

Uniprot Description

MAP2: a member of the microtubule-associated protein (MAP) family. Its exact function is unknown but MAPs appear to stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Expressed at high levels in neural tissue and the prostate. Four alternatively spliced transcript variants have been reported.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q34-q35

Cellular Component: microtubule; microtubule associated complex; cell soma; cytoplasm; nucleolus; dendritic shaft

Molecular Function: calmodulin binding; protein binding; microtubule binding; structural molecule activity

Biological Process: axonogenesis; dendrite morphogenesis; microtubule bundle formation; neurite development; central nervous system neuron development