Monkey EPHA10 ELISA Kit | EPHA10 elisa kit

Monkey EPHA10 (Ephrin Type A Receptor 10) ELISA Kit

Cat. Num. AAA2505124

• Reactivity: Monkey
• Synonyms: EPHA10; Monkey EPHA10 (Ephrin Type A Receptor 10) ELISA Kit; EPHA10 elisa kit

• Reactivity: Monkey
• Specificity: This kit recognizes natural and recombinant Monkey EPHA10. No significant cross-reactivity or interference between Monkey EPHA10 and analogues was observed.
• Sequence Length: 295

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Related Product Information for EPHA10 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey EPHA10 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to EPHA10. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for EPHA10 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain EPHA10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of EPHA10. You can calculate the concentration of EPHA10 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 150456469
• NCBI GeneID: 284656
• NCBI Accession #: NP_775912.2
• NCBI GenBank Nucleotide #: NM_173641.2
• UniProt Accession #: Q5JZY3; Q6NW42; A4FU89; J3KPB5
• Molecular Weight: 97,293 Da
• NCBI Official Full Name: ephrin type-A receptor 10 isoform 2
• NCBI Official Synonym Full Names: EPH receptor A10
• NCBI Official Symbol: EPHA10
• NCBI Protein Information: ephrin type-A receptor 10; EphA10s protein
• UniProt Protein Name: Ephrin type-A receptor 10
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA10
• UniProt Entry Name: EPHAA_HUMAN

NCBI Description

Ephrin receptors, the largest subfamily of receptor tyrosine kinases (RTKs), and their ephrin ligands are important mediators of cell-cell communication regulating cell attachment, shape, and mobility in neuronal and epithelial cells (Aasheim et al., 2005 [PubMed 15777695]). See MIM 179610 for additional background on Eph receptors and ephrins.[supplied by OMIM, Mar 2008]

Uniprot Description

EphA10: Receptor for members of the ephrin-A family. Binds to EFNA3, EFNA4 and EFNA5. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (receptor); Membrane protein, integral; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; transmembrane-ephrin receptor activity; ephrin receptor activity; ATP binding

Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; ephrin receptor signaling pathway