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Typical Testing Data/Standard Curve (for reference only)

Human Ephrin Type A Receptor 10 (EPHA10) ELISA Kit | EPHA10 elisa kit

Human Ephrin Type A Receptor 10 (EPHA10) ELISA Kit

Reactivity
Human
Synonyms
Ephrin Type A Receptor 10 (EPHA10); Human Ephrin Type A Receptor 10 (EPHA10) ELISA Kit; EPHA10 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Ephrin Type A Receptor 10 (EPHA10).
No significant cross-reactivity or interference between Ephrin Type A Receptor 10 (EPHA10) and analogues was observed.
Sequence Length
1008
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates and Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.109ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Ephrin Type A Receptor 10 (EPHA10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Ephrin Type A Receptor 10 (EPHA10) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for EPHA10 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin Type A Receptor 10 (EPHA10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin Type A Receptor 10 (EPHA10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin Type A Receptor 10 (EPHA10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ephrin Type A Receptor 10 (EPHA10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for EPHA10 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
97,293 Da
NCBI Official Full Name
ephrin type-A receptor 10 isoform 3
NCBI Official Synonym Full Names
EPH receptor A10
NCBI Official Symbol
EPHA10
NCBI Protein Information
ephrin type-A receptor 10
UniProt Protein Name
Ephrin type-A receptor 10
Protein Family
UniProt Gene Name
EPHA10
UniProt Entry Name
EPHAA_HUMAN

NCBI Description

Ephrin receptors, the largest subfamily of receptor tyrosine kinases (RTKs), and their ephrin ligands are important mediators of cell-cell communication regulating cell attachment, shape, and mobility in neuronal and epithelial cells (Aasheim et al., 2005 [PubMed 15777695]). See MIM 179610 for additional background on Eph receptors and ephrins.[supplied by OMIM, Mar 2008]

Uniprot Description

EphA10: Receptor for members of the ephrin-A family. Binds to EFNA3, EFNA4 and EFNA5. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; Membrane protein, integral; TK group; Eph family

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; transmembrane-ephrin receptor activity; ephrin receptor activity; ATP binding

Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; ephrin receptor signaling pathway

Research Articles on EPHA10

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Product Notes

The Human EPHA10 epha10 (Catalog #AAA2020818) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2020818 ELISA Kit recognizes Human EPHA10. It is sometimes possible for the material contained within the vial of "Ephrin Type A Receptor 10 (EPHA10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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