Monkey ELA2B ELISA Kit | ELA2B elisa kit

Monkey ELA2B (Elastase 2B) ELISA Kit

Cat. Num. AAA2509410

• Gene Names: CELA2B; ELA2B
• Reactivity: Monkey
• Synonyms: ELA2B; Monkey ELA2B (Elastase 2B) ELISA Kit; ELA2B elisa kit

• Reactivity: Monkey
• Specificity: This kit recognizes natural and recombinant Monkey ELA2B. No significant cross-reactivity or interference between Monkey ELA2B and analogues was observed.
• Sequence Length: 269

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Related Product Information for ELA2B elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey ELA2B concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ELA2B. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ELA2B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ELA2B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ELA2B. You can calculate the concentration of ELA2B in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119572113
• NCBI GeneID: 51032
• UniProt Accession #: P08218; Q14D16; Q6ISM5; Q96QV5
• Molecular Weight: 28,810 Da
• NCBI Official Full Name: elastase 2B
• NCBI Official Synonym Full Names: chymotrypsin-like elastase family, member 2B
• NCBI Official Symbol: CELA2B
• NCBI Official Synonym Symbols: ELA2B
• NCBI Protein Information: chymotrypsin-like elastase family member 2B; RP11-265F14.2; elastase 2B; elastase-2B; pancreatic elastase IIB (ELA2B)
• UniProt Protein Name: Chymotrypsin-like elastase family member 2B
• UniProt Gene Name: CELA2B
• UniProt Synonym Gene Names: ELA2B
• UniProt Entry Name: CEL2B_HUMAN

NCBI Description

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2B is secreted from the pancreas as a zymogen. In other species, elastase 2B has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. [provided by RefSeq, Jul 2008]

Uniprot Description

CELA2B: Acts upon elastin. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: EC 3.4.21.71; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase activity

Biological Process: proteolysis