Monkey CDK6 ELISA Kit | CDK6 elisa kit

Monkey CDK6 (Cyclin Dependent Kinase 6) ELISA Kit

Cat. Num. AAA2508448

• Gene Names: CDK6; MCPH12; PLSTIRE
• Reactivity: Monkey
• Synonyms: CDK6; Monkey CDK6 (Cyclin Dependent Kinase 6) ELISA Kit; CDK6 elisa kit

• Reactivity: Monkey
• Specificity: This kit recognizes natural and recombinant Monkey CDK6. No significant cross-reactivity or interference between Monkey CDK6 and analogues was observed.
• Sequence Length: 326

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Related Product Information for CDK6 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey CDK6 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CDK6. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CDK6 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CDK6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CDK6. You can calculate the concentration of CDK6 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502741
• NCBI GeneID: 1021
• NCBI Accession #: NP_001250.1
• NCBI GenBank Nucleotide #: NM_001259.6
• UniProt Accession #: Q00534; A4D1G0
• Molecular Weight: 36,938 Da
• NCBI Official Full Name: cyclin-dependent kinase 6
• NCBI Official Synonym Full Names: cyclin-dependent kinase 6
• NCBI Official Symbol: CDK6
• NCBI Official Synonym Symbols: MCPH12; PLSTIRE
• NCBI Protein Information: cyclin-dependent kinase 6; cell division protein kinase 6; serine/threonine-protein kinase PLSTIRE
• UniProt Protein Name: Cyclin-dependent kinase 6
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK6
• UniProt Synonym Gene Names: CDKN6
• UniProt Entry Name: CDK6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]

Uniprot Description

CDK6: a protein kinase of the CDK family. Important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. Phosphorylates and regulates the activity of the Rb tumor suppressor protein. Overexpressed and/or disrupted by translocation in leukemias, lymphomas and other cancers and amplified in gliomas and rodent cancers.

Protein type: Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.22; Kinase, protein; Cell cycle regulation; CMGC group; CDK family; CDK4 subfamily; CDK/CDK4 subfamily

Chromosomal Location of Human Ortholog: 7q21-q22

Cellular Component: nucleoplasm; ruffle; centrosome; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; cytosol

Molecular Function: cyclin binding; protein binding; cyclin-dependent protein kinase activity; ATP binding

Biological Process: Notch signaling pathway; dentate gyrus development; negative regulation of myeloid cell differentiation; response to virus; gliogenesis; T cell differentiation in the thymus; astrocyte development; protein amino acid phosphorylation; negative regulation of cell cycle; regulation of erythrocyte differentiation; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; negative regulation of cell differentiation; regulation of gene expression; generation of neurons; cell division; cell dedifferentiation; negative regulation of osteoblast differentiation; mitotic cell cycle; positive regulation of cell-matrix adhesion; cell cycle arrest; lateral ventricle development; negative regulation of epithelial cell proliferation; G1/S transition of mitotic cell cycle

Disease: Microcephaly 12, Primary, Autosomal Recessive