Monkey Beta-klotho (KLB) ELISA Kit | KLB elisa kit
Monkey Beta-klotho (KLB) ELISA Kit
Gene Names
Klb; AV071179; betaKlotho
Reactivity
Monkey
Synonyms
Beta-klotho (KLB); Monkey Beta-klotho (KLB) ELISA Kit; KLB elisa kit
Reactivity
Monkey
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Standard Curve/Testing Data
Related Product Information for KLB elisa kit
Intended Uses: This KLB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey KLB. This ELISA kit for research use only!
Principle of the Assay: KLB ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-KLB antibody and an KLB-HRP conjugate. The assay sample and buffer are incubated together with KLB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLB concentration since KLB from samples and KLB-HRP conjugate compete for the anti-KLB antibody binding site. Since the number of sites is limited, as more sites are occupied by KLB from the sample, fewer sites are left to bind KLB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLB concentration in each sample is interpolated from this standard curve.
Principle of the Assay: KLB ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-KLB antibody and an KLB-HRP conjugate. The assay sample and buffer are incubated together with KLB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLB concentration since KLB from samples and KLB-HRP conjugate compete for the anti-KLB antibody binding site. Since the number of sites is limited, as more sites are occupied by KLB from the sample, fewer sites are left to bind KLB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLB concentration in each sample is interpolated from this standard curve.
Product Categories/Family for KLB elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,142 Da
NCBI Official Full Name
beta-klotho
NCBI Official Synonym Full Names
klotho beta
NCBI Official Symbol
Klb
NCBI Official Synonym Symbols
AV071179; betaKlotho
NCBI Protein Information
beta-klotho; BKL; klotho beta-like protein
UniProt Protein Name
Beta-klotho
Protein Family
UniProt Gene Name
Klb
UniProt Synonym Gene Names
Betakl; BKL; BetaKlotho
UniProt Entry Name
KLOTB_MOUSE
Uniprot Description
Klotho beta: Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
Protein type: Membrane protein, integral
Cellular Component: membrane; integral to membrane; plasma membrane
Molecular Function: fibroblast growth factor binding; fibroblast growth factor receptor binding; hydrolase activity, hydrolyzing O-glycosyl compounds
Biological Process: fibroblast growth factor receptor signaling pathway; carbohydrate metabolic process; positive regulation of cell proliferation
