Human matrix metallopeptidase 8 (neutrophil collagenase) ELISA Kit | MMP8 elisa kit

Human Matrix metalloproteinase 8/Neutrophil collagenase, MMP-8 ELISA Kit

Cat. Num. AAA702847

• Gene Names: MMP8; HNC; CLG1; MMP-8; PMNL-CL
• Reactivity: Human
• Synonyms: matrix metallopeptidase 8 (neutrophil collagenase); Human Matrix metalloproteinase 8/Neutrophil collagenase; MMP-8 ELISA Kit; MMP-8 ELISA kit; CLG1; HNC; PMNL-CL; PMNL collagenase; matrix metalloproteinase 8; matrix metalloproteinase 8 (neutrophil collagenase) ; MMP8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human MMP-8. No significant cross-reactivity or interference between human MMP-8 and analogues was observed.
• Sequence Length: 467

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 39 pg/ml-2500 pg/ml
• Sensitivity: < 9.75 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP8 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4505221
• NCBI GeneID: 4317
• NCBI Accession #: NP_002415.1
• NCBI GenBank Nucleotide #: NM_002424.2
• UniProt Accession #: P22894; Q45F99
• Molecular Weight: 53,412 Da
• NCBI Official Full Name: neutrophil collagenase preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 8 (neutrophil collagenase)
• NCBI Official Symbol: MMP8
• NCBI Official Synonym Symbols: HNC; CLG1; MMP-8; PMNL-CL
• NCBI Protein Information: neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase)
• UniProt Protein Name: Neutrophil collagenase
• Protein Family: Neutrophil collagenase
• UniProt Gene Name: MMP8
• UniProt Synonym Gene Names: CLG1; MMP-8; PMNL-CL
• UniProt Entry Name: MMP8_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Can degrade fibrillar type I, II, and III collagens.

Catalytic activity: Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7, this enzyme cleaves type III collagen more slowly than type I.

Cofactor: Binds 3 calcium ions per subunit.

Enzyme regulation: Cannot be activated without removal of the activation peptide.