Mouse matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) ELISA Kit | MMP2 elisa kit

Mouse matrix metalloproteinase 2/Gelatinase A, MMP-2 ELISA Kit

Cat. Num. AAA700408

• Gene Names: Mmp2; GelA; Clg4a; MMP-2
• Reactivity: Mouse
• Synonyms: matrix metallopeptidase 2 (gelatinase A; 72kDa gelatinase; 72kDa type IV collagenase); Mouse matrix metalloproteinase 2/Gelatinase A; MMP-2 ELISA Kit; MMP-2 ELISA kit; CLG4; CLG4A; MMP-II; MONA; TBE-1; collagenase type IV-A; matrix metalloproteinase 2; matrix metalloproteinase-II; neutrophil gelatinase; MMP2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse MMP2. No significant cross-reactivity or interference between Mouse MMP2 and analogues was observed.
• Sequence Length: 662

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: 3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Angiogenesis
• Protein Biological Process 3: Angiogenesis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP2 elisa kit

Introduction: 72 kDa type IV collagenase also known as matrixmetalloproteinase-2 (MMP-2) and gelatinase A is an enzyme thatin humans is encoded by the MMP2 gene.Proteins of the matrix metalloproteinase (MMP) family are involvedin the breakdown of extracellular matrix in normal physiologicalprocesses, such as embryonic development, reproduction, andtissue remodeling, as well as in disease processes, such asarthritis and metastasis. Most MMP's are secreted as inactiveproproteins which are activated when cleaved by extracellularproteinases. This gene encodes an enzyme which degrades typeIV collagen, the major structural component of basementmembranes. The enzyme plays a role in endometrial menstrualbreakdown, regulation of vascularization and the inflammatoryresponse

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-2. Standards or samples are then addedto the appropriate microtiter plate wells with a biotin-conjugatedantibody preparation specific for MMP-2 and Avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate welland incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine)substrate solution is added to each well. Only those wells thatcontain MMP-2, biotin-conjugated antibody andenzyme-conjugated Avidin will exhibit a change in color. Theenzyme-substrate reaction is terminated by the addition of asulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of MMP-2 in the samples is then determined bycomparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6678902
• NCBI GeneID: 17390
• NCBI Accession #: NP_032636.1
• NCBI GenBank Nucleotide #: NM_008610.2
• UniProt Accession #: P33434
• Molecular Weight: 74,102 Da
• NCBI Official Full Name: 72 kDa type IV collagenase
• NCBI Official Synonym Full Names: matrix metallopeptidase 2
• NCBI Official Symbol: Mmp2
• NCBI Official Synonym Symbols: GelA; Clg4a; MMP-2
• NCBI Protein Information: 72 kDa type IV collagenase; gelatinase A; 72kD gelatinase; 72kDa gelatinase; 72 kDa gelatinase; 72kD type IV collagenase; 72kDa type IV collagenase; matrix metalloproteinase 2; matrix metalloproteinase-2
• UniProt Protein Name: 72 kDa type IV collagenase
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: Mmp2
• UniProt Synonym Gene Names: MMP-2
• UniProt Entry Name: MMP2_MOUSE

Uniprot Description

Function: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues 1 PublicationManual assertion based on experiment in:Ref.4

Catalytic activity: Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.

Cofactor: Binds 4 calcium ions per subunit