Rabbit matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) ELISA Kit | MMP2 elisa kit

Rabbit matrix metalloproteinase 2/Gelatinase A, MMP-2 ELISA Kit

Cat. Num. AAA701436

• Reactivity: Rabbit
• Synonyms: matrix metallopeptidase 2 (gelatinase A; 72kDa gelatinase; 72kDa type IV collagenase); Rabbit matrix metalloproteinase 2/Gelatinase A; MMP-2 ELISA Kit; CLG4; CLG4A; MMP-II; MONA; TBE-1; collagenase type IV-A; matrix metalloproteinase 2; matrix metalloproteinase-II; neutrophil gelatinase; MMP2 elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of rabbit MMP-2. No significant cross-reactivity or interference between rabbit MMP-2 and analogues was observed.
• Sequence Length: 662

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich
• Detection Range: 1.56 ng/ml -100 ng/ml.
• Sensitivity: 0.39 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP2 elisa kit

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 126722977
• NCBI GeneID: 100009000
• NCBI Accession #: NP_001075678.1
• NCBI GenBank Nucleotide #: NM_001082209.1
• UniProt Accession #: P50757
• Molecular Weight: 73,803 Da
• NCBI Official Full Name: 72 kDa type IV collagenase
• NCBI Official Symbol: MMP2
• NCBI Protein Information: 72 kDa type IV collagenase; MMP-2; gelatinase A; 72 kDa gelatinase; matrix metalloproteinase-2
• UniProt Protein Name: 72 kDa type IV collagenase
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP2
• UniProt Synonym Gene Names: MMP-2
• UniProt Entry Name: MMP2_RABIT

Uniprot Description

Function: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues

Catalytic activity: Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.

Cofactor: Binds 4 calcium ions per subunit