Rabbit anti-Human DDX43 Polyclonal Antibody | anti-DDX43 antibody

DDX43 Antibody

Cat. Num. AAA9412968

• Gene Names: DDX43; CT13; HAGE
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: DDX43; Polyclonal Antibody; DDX43 Antibody; CT13; HAGE; anti-DDX43 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total DDX43 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 2.3 mg/ml (varies by lot)
• Sequence Length: 648

• Applicable Applications for anti-DDX43 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:200-1:1000; Immunohistochemistry: 1:50-1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Fusion protein corresponding to a region derived from internal residues of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 43
• Target Name: DDX43
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 8%SDS-PAGE Lysate: 40ug HepG2 cell Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/50.)

Related Product Information for anti-DDX43 antibody

DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis and cellular growth and division. DDX43 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 43), also known as CT13 or HAGE, is a 648 amino acid protein that contains one KH domain, one helicase C-terminal domain and one helicase ATP-binding domain and belongs to the DEAD-box family. Expressed in testis and present at abnormally high levels in a variety of tumors, DDX43 is thought to function as an ATP-dependent RNA helicase that may play a role tumor transformation and metastasis.

Product Categories/Family for anti-DDX43 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 222352149
• NCBI GeneID: 55510
• NCBI Accession #: NP_061135.2
• NCBI GenBank Nucleotide #: NM_018665.2
• UniProt Accession #: Q9NXZ2; Q6NXR1; B4E0C8
• Molecular Weight: 21,295 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX43
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 43
• NCBI Official Symbol: DDX43
• NCBI Official Synonym Symbols: CT13; HAGE
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX43
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX43
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX43
• UniProt Synonym Gene Names: HAGE; CT13
• UniProt Entry Name: DDX43_HUMAN

NCBI Description

The protein encoded by this gene is an ATP-dependent RNA helicase in the DEAD-box family and displays tumor-specific expression. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX43: is an ATP-dependent RNA helicase in the DEAD-box family and displays tumor-specific expression. [provided by RefSeq, Jul 2008]

Protein type: Cancer Testis Antigen (CTA); EC 3.6.4.13; Helicase; RNA-binding

Chromosomal Location of Human Ortholog: 6q13

Cellular Component: intracellular

Molecular Function: RNA binding; ATP-dependent RNA helicase activity; ATP binding

Biological Process: metabolic process