Human Promotilin ELISA Kit | MLN elisa kit

Human Promotilin ELISA Kit

Cat. Num. AAA766018

• Reactivity: Human
• Synonyms: Promotilin; Human Promotilin ELISA Kit; MLN (Promotilin); MLN elisa kit

• Reactivity: Human
• Sequence Length: 108

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MLN elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human MLN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MLN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MLN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MLN can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 296010792
• NCBI GeneID: 4295
• NCBI Accession #: NP_001171627.1
• NCBI GenBank Nucleotide #: NM_001184698.1
• Molecular Weight: 12,849 Da
• NCBI Official Full Name: promotilin isoform 3 preproprotein
• NCBI Official Synonym Full Names: motilin
• NCBI Official Symbol: MLN
• NCBI Protein Information: promotilin
• UniProt Protein Name: Promotilin
• Protein Family: Promotilin
• UniProt Gene Name: MLN
• UniProt Synonym Gene Names: MAP
• UniProt Entry Name: MOTI_HUMAN

NCBI Description

This gene encodes a small peptide hormone that is secreted by cells of the small intestine to regulate gastrointestinal contractions and motility. Proteolytic processing of the secreted protein produces the mature peptide and a byproduct referred to as motilin-associated peptide (MAP). Three transcript variants encoding different preproprotein isoforms but the same mature peptide have been found for this gene. [provided by RefSeq, May 2010]

Uniprot Description

MLN: Plays an important role in the regulation of interdigestive gastrointestinal motility and indirectly causes rhythmic contraction of duodenal and colonic smooth muscle. Belongs to the motilin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular region

Molecular Function: hormone activity; receptor binding

Biological Process: cell-cell signaling; G-protein coupled receptor protein signaling pathway