Rabbit anti-Human Proteasome 26S Subunit, Non ATPase 7 (PSMD7) Polyclonal Antibody | anti-PSMD7 antibody

Polyclonal Antibody to Proteasome 26S Subunit, Non ATPase 7 (PSMD7)

Cat. Num. AAA2016477

• Gene Names: PSMD7; P40; S12; Rpn8; MOV34
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Proteasome 26S Subunit; Non ATPase 7 (PSMD7); Polyclonal Antibody; Polyclonal Antibody to Proteasome 26S Subunit; anti-PSMD7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against PSMD7. It has been selected for its ability to recognize PSMD7 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MPELAVQKVV VHPLVLLSVV DHFNRIGKVG NQKRVVGVLL GSWQKKVLDV SNSFAVPFDE DDKDDSVWFL DHDYLENMYG MFKKVNARER IVGWYHTGPK LHKNDIAINE LMKRYCPNSV LVIIDVKPKD LGLPTEAYIS VEEVHDDGTP TSKTFEHVTS EIGAEEAEEV GVEHLLRDIK DTTVGTLSQR ITNQVHGLKG LNSKLLDIRS YLEKVATGKL PINHQIIYQL QDVFNLLPDV SLQEFVKAFY LKTNDQMVVV YLASLIRSVV ALHNLINNKI ANRDAEKKEG QEKEESKKDR KEDKEKDKDK

• Applicable Applications for anti-PSMD7 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant PSMD7 (Met1~Lys324) expressed in E.coli.
• Cross Reactivity: Human
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2077726
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human 293T Cells; Lane3: Human Liver Tissue; Lane4: Human Lung Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant PSMD7, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Human Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 25777615
• NCBI GeneID: 5713
• NCBI Accession #: NP_002802.2
• NCBI GenBank Nucleotide #: NM_002811.4
• UniProt Accession #: P51665; Q6PKI2; Q96E97; D3DWS9
• Molecular Weight: 37,025 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 7
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 7
• NCBI Official Symbol: PSMD7
• NCBI Official Synonym Symbols: P40; S12; Rpn8; MOV34
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 7
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 7
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD7
• UniProt Synonym Gene Names: MOV34L

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMD7: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Belongs to the peptidase M67A family.

Protein type: Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: cytosol; extracellular exosome; extracellular region; ficolin-1-rich granule lumen; membrane; nucleoplasm; nucleus; proteasome complex; proteasome regulatory particle; secretory granule lumen

Molecular Function: protein binding; protein homodimerization activity

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; Fc-epsilon receptor signaling pathway; MAPK cascade; negative regulation of canonical Wnt signaling pathway; negative regulation of G2/M transition of mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; neutrophil degranulation; NIK/NF-kappaB signaling; positive regulation of canonical Wnt signaling pathway; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; post-translational protein modification; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; protein polyubiquitination; regulation of cellular amino acid metabolic process; regulation of hematopoietic stem cell differentiation; regulation of mRNA stability; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; transmembrane transport; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway