Typical Testing Data/Standard Curve (for reference only)
Rabbit Motilin ELISA Kit | MTL elisa kit
Rabbit Motilin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: MTL ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MTL antibody and an MTL-HRP conjugate. The assay sample and buffer are incubated together with MTL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MTL concentration since MTL from samples and MTL-HRP conjugate compete for the anti-MTL antibody binding site. Since the number of sites is limited, as more sites are occupied by MTL from the sample, fewer sites are left to bind MTL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MTL concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a small peptide hormone that is secreted by cells of the small intestine to regulate gastrointestinal contractions and motility. Proteolytic processing of the secreted protein produces the mature peptide and a byproduct referred to as motilin-associated peptide (MAP). Three transcript variants encoding different preproprotein isoforms but the same mature peptide have been found for this gene. [provided by RefSeq, May 2010]
Uniprot Description
MLN: Plays an important role in the regulation of interdigestive gastrointestinal motility and indirectly causes rhythmic contraction of duodenal and colonic smooth muscle. Belongs to the motilin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: extracellular region
Molecular Function: hormone activity; receptor binding
Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling
Research Articles on MTL
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Product Notes
The Rabbit MTL mln (Catalog #AAA732745) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA732745 ELISA Kit recognizes Rabbit MTL. It is sometimes possible for the material contained within the vial of "Motilin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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