E3 ubiquitin-protein ligase makorin-1 (MKRN1) Recombinant Protein | MKRN1 recombinant protein

Recombinant Human E3 ubiquitin-protein ligase makorin-1 (MKRN1)

Cat. Num. AAA1335322

• Gene Names: MKRN1; RNF61
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: E3 ubiquitin-protein ligase makorin-1 (MKRN1); Recombinant Human E3 ubiquitin-protein ligase makorin-1 (MKRN1); MKRN1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-482, Full length protein
• Sequence: MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEEKEEKQKLILKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTSSRYRAQRRNHFWELIEERENSNPFDNDEEEVVTFELGEMLLMLLAAGGDDELTDSEDEWDLFHDELEDFYDLDL
• Sequence Length: 482

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 223468622
• NCBI GeneID: 23608
• NCBI Accession #: NP_001138597.1
• NCBI GenBank Nucleotide #: NM_001145125.1
• UniProt Accession #: Q9UHC7; Q256Y7; Q59G11; Q6GSF1; Q9H0G0; Q9UEZ7; Q9UHW2; A4D1T7; B3KXB4
• Molecular Weight: 35,224 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase makorin-1 isoform 2
• NCBI Official Synonym Full Names: makorin ring finger protein 1
• NCBI Official Symbol: MKRN1
• NCBI Official Synonym Symbols: RNF61
• NCBI Protein Information: E3 ubiquitin-protein ligase makorin-1
• UniProt Protein Name: E3 ubiquitin-protein ligase makorin-1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: MKRN1
• UniProt Synonym Gene Names: RNF61

NCBI Description

This gene encodes a protein that belongs to a novel class of zinc finger proteins. The encoded protein functions as a transcriptional co-regulator, and as an E3 ubiquitin ligase that promotes the ubiquitination and proteasomal degradation of target proteins. The protein encoded by this gene is thought to regulate RNA polymerase II-catalyzed transcription. Substrates for this protein's E3 ubiquitin ligase activity include the capsid protein of the West Nile virus and the catalytic subunit of the telomerase ribonucleoprotein. This protein controls cell cycle arrest and apoptosis by regulating p21, a cell cycle regulator, and the tumor suppressor protein p53. Pseudogenes of this gene are present on chromosomes 1, 3, 9, 12 and 20, and on the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014]

Uniprot Description

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.