Mouse Macrophage migration inhibitory factor ELISA Kit | Mif elisa kit

Mouse Macrophage migration inhibitory factor ELISA Kit

Cat. Num. AAA764898

• Gene Names: MIF; GIF; GLIF; MMIF
• Reactivity: Mouse
• Synonyms: Macrophage migration inhibitory factor; Mouse Macrophage migration inhibitory factor ELISA Kit; Mif (Macrophage migration inhibitory factor)/GIFmacrophage migration inhibitory factor/GLIF/Glycosylation-inhibiting factor/L-dopachrome isomerase/L-dopachrome tautomerase/macrophage migration inhibitory factor (glycosylation-inhibiting factor)/MMIF/Phenylpyruvate tautomerase; Mif elisa kit

• Reactivity: Mouse
• Sequence Length: 115

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Mif elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Mif antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Mif antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Mif amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Mif can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4505185
• NCBI GeneID: 4282
• NCBI Accession #: NP_002406.1
• NCBI GenBank Nucleotide #: NM_002415.1
• Molecular Weight: 12,476 Da
• NCBI Official Full Name: macrophage migration inhibitory factor
• NCBI Official Synonym Full Names: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• NCBI Official Symbol: MIF
• NCBI Official Synonym Symbols: GIF; GLIF; MMIF
• NCBI Protein Information: macrophage migration inhibitory factor
• UniProt Protein Name: Macrophage migration inhibitory factor
• Protein Family: Macrophage migration inhibitory factor
• UniProt Gene Name: MIF
• UniProt Synonym Gene Names: GLIF; MMIF; MIF; GIF
• UniProt Entry Name: MIF_HUMAN

NCBI Description

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]

Uniprot Description

MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.

Protein type: EC 5.3.3.12; Cytokine; Cell development/differentiation; Apoptosis; Amino Acid Metabolism - tyrosine; Isomerase; Amino Acid Metabolism - phenylalanine; EC 5.3.2.1

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; myelin sheath; nucleoplasm; vesicle

Molecular Function: chemoattractant activity; cytokine activity; dopachrome isomerase activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; phenylpyruvate tautomerase activity; protein binding; receptor binding

Biological Process: blood coagulation; carboxylic acid metabolic process; cell aging; cell proliferation; cell surface receptor linked signal transduction; DNA damage response, signal transduction by p53 class mediator; inflammatory response; innate immune response; leukocyte migration; negative regulation of apoptosis; negative regulation of cellular protein metabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of mature B cell apoptosis; negative regulation of myeloid cell apoptosis; positive chemotaxis; positive regulation of B cell proliferation; positive regulation of cytokine secretion; positive regulation of fibroblast proliferation; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; prostaglandin biosynthetic process; regulation of macrophage activation

Disease: Rheumatoid Arthritis, Systemic Juvenile