METAP1D recombinant protein

METAP1D, 20-335aa, Human, His tag, E Coli

Cat. Num. AAA204921

• Gene Names: METAP1D; MAP1D; MAP 1D; Metap1l; MetAP 1D
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: METAP1D; 20-335aa; Human; His tag; E Coli; Methionine aminopeptidase 1D mitochondrial precursor; Methionine aminopeptidase 1D; mitochondrial precursor; MAP1D; Metap1l; METAP1D recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT
• Concentration: 0.25mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSSSPLNHI YLHKQSSSQQ RRNFFFRRQR DISHSIVLPA AVSSAHPVPK HIKKPDYVTT GIVPDWGDSI EVKNEDQIQG LHQACQLARH VLLLAGKSLK VDMTTEEIDA LVHREIISHN AYPSPLGYGG FPKSVCTSVN NVLCHGIPDS RPLQDGDIIN IDVTVYYNGY HGDTSETFLV GNVDECGKKL VEVARRCRDE AIAACRAGAP FSVIGNTISH ITHQNGFQVC PHFVGHGIGS YFHGHPEIWH HANDSDLPME EGMAFTIEPI ITEGSPEFKV LEDAWTVVSL DNQRSAQFEH TVLITSRGAQ ILTKLPHEA
• Sequence Length: 335

• Applicable Applications for METAP1D recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for METAP1D recombinant protein

Methionine aminopeptidase 1D, mitochondrial precursor, also known as METAP1D, is a 335 amino acid mitochondrial protein that belongs to the peptidase M24A family. It is overexpressed in colon cancer cell lines, suggesting a role in tumorigenesis. METAP1D has also been found to remove methionine from the N-terminus of nascent proteins. METAP1D binds two cobalt ions per subunit and is encoded by a gene that maps to human chromosome 2q31.1. Recombinant human METAP1D protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for METAP1D recombinant protein

Immunology

References

Leszczyniecka M., et al. (2006) Oncogene. 25: 3471-3478.; Serero A., et al. (2003) J Biol Chem. 278: 52953-52963.;

NCBI and Uniprot Product Information

• NCBI GI #: 40385867
• NCBI GeneID: 254042
• NCBI Accession #: NP_954697
• NCBI GenBank Nucleotide #: NM_199227.2
• UniProt Accession #: Q6UB28; Q1WNX3
• Molecular Weight: 37.4 kDa (339aa) confirmed by MALDI-TOF
• NCBI Official Full Name: methionine aminopeptidase 1D, mitochondrial isoform 1
• NCBI Official Synonym Full Names: methionyl aminopeptidase type 1D, mitochondrial
• NCBI Official Symbol: METAP1D
• NCBI Official Synonym Symbols: MAP1D; MAP 1D; Metap1l; MetAP 1D
• NCBI Protein Information: methionine aminopeptidase 1D, mitochondrial
• UniProt Protein Name: Methionine aminopeptidase 1D, mitochondrial
• UniProt Gene Name: METAP1D
• UniProt Synonym Gene Names: MAP1D; MAP 1D; MetAP 1D

NCBI Description

The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).[supplied by OMIM, Mar 2008]

Uniprot Description

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed (). May play a role in colon tumorigenesis.