Human major histocompatibility complex class I chain-related gene A, MICA ELISA Kit | MICA elisa kit

Human major histocompatibility complex class I chain-related gene A, MICA ELISA Kit

Cat. Num. AAA163367

• Gene Names: MICA; MIC-A; PERB11.1
• Reactivity: Human
• Synonyms: major histocompatibility complex class I chain-related gene A; MICA; Human major histocompatibility complex class I chain-related gene A; MICA ELISA Kit; MICA elisa kit

• Reactivity: Human
• Sequence Length: 317

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 5pg/ml - 2000pg/ml
• Sensitivity: 2.48pg/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MICA elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Major Histocompatibility Complex Class I Chain-related Gene A (also known as MICA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human MICA antibody. MICA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human MICA Antibody is added and binds to MICA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MICA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human MICA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 327505520
• NCBI GeneID: 100507436
• Molecular Weight: 31,577 Da
• NCBI Official Full Name: major histocompatibility complex class I chain-related protein A, partial
• NCBI Official Synonym Full Names: MHC class I polypeptide-related sequence A
• NCBI Official Symbol: MICA
• NCBI Official Synonym Symbols: MIC-A; PERB11.1
• NCBI Protein Information: MHC class I polypeptide-related sequence A
• UniProt Protein Name: MHC class I polypeptide-related sequence A
• Protein Family: Microperfuranone synthase
• UniProt Gene Name: MICA
• UniProt Synonym Gene Names: MIC-A

NCBI Description

This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.