Rat Matrix Gla Protein ELISA Kit | MGP elisa kit

Rat Matrix Gla Protein ELISA Kit

Cat. Num. AAA723133

• Gene Names: MGP; NTI; GIG36; MGLAP
• Reactivity: Rat
• Synonyms: Matrix Gla Protein; Rat Matrix Gla Protein ELISA Kit; MGP elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/ml
• Intended Uses: This MGP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MGP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MGP elisa kit

Principle of the Assay: MGP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MGP. Standards or samples are then added to the microtiter plate wells and MGP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MGP present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for MGP are added to each well to "sandwich" the MGP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MGP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MGP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MGP elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 47496663
• NCBI GeneID: 4256
• UniProt Accession #: P08493; Q2TU41; Q567P9; Q6ICN5; A0M8W5; B2R519; J3KMX7
• Molecular Weight: 15,325 Da
• NCBI Official Full Name: MGP, partial
• NCBI Official Synonym Full Names: matrix Gla protein
• NCBI Official Symbol: MGP
• NCBI Official Synonym Symbols: NTI; GIG36; MGLAP
• NCBI Protein Information: matrix Gla protein; cell growth-inhibiting gene 36 protein
• UniProt Protein Name: Matrix Gla protein
• Protein Family: Matrix Gla protein
• UniProt Gene Name: MGP
• UniProt Synonym Gene Names: MGLAP; GIG36; MGP
• UniProt Entry Name: MGP_HUMAN

NCBI Description

The protein encoded by this gene is secreted and likely acts as an inhibitor of bone formation. The encoded protein is found in the organic matrix of bone and cartilage. Defects in this gene are a cause of Keutel syndrome (KS). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

MGP: Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation. Defects in MGP are the cause of Keutel syndrome (KS). KS is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia. Belongs to the osteocalcin/matrix Gla protein family.

Protein type: Secreted, signal peptide; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: protein binding; structural constituent of bone; extracellular matrix structural constituent; calcium ion binding

Biological Process: ossification; cell differentiation; regulation of bone mineralization; cartilage condensation

Disease: Keutel Syndrome