Mouse Matrix Gla protein ELISA Kit | Mgp elisa kit

Mouse Matrix Gla protein ELISA Kit

Cat. Num. AAA760987

• Gene Names: MGP; NTI; GIG36; MGLAP
• Reactivity: Mouse
• Synonyms: Matrix Gla protein; Mouse Matrix Gla protein ELISA Kit; Mgp/Matrix Gla Protein/Cell growth-inhibiting gene 36 protein; Mgp elisa kit

• Reactivity: Mouse
• Sequence Length: 103

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Mgp elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Mgp antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Mgp antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Mgp amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Mgp can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 187591
• NCBI GeneID: 4256
• Molecular Weight: 15,325 Da
• NCBI Official Full Name: matrix Gla protein
• NCBI Official Synonym Full Names: matrix Gla protein
• NCBI Official Symbol: MGP
• NCBI Official Synonym Symbols: NTI; GIG36; MGLAP
• NCBI Protein Information: matrix Gla protein
• UniProt Protein Name: Matrix Gla protein
• Protein Family: Matrix Gla protein
• UniProt Gene Name: MGP
• UniProt Synonym Gene Names: MGLAP; MGP
• UniProt Entry Name: MGP_HUMAN

NCBI Description

The protein encoded by this gene is secreted and likely acts as an inhibitor of bone formation. The encoded protein is found in the organic matrix of bone and cartilage. Defects in this gene are a cause of Keutel syndrome (KS). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

MGP: Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation. Defects in MGP are the cause of Keutel syndrome (KS). KS is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia. Belongs to the osteocalcin/matrix Gla protein family.

Protein type: Secreted; Extracellular matrix; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; extracellular matrix structural constituent; protein binding; structural constituent of bone

Biological Process: cartilage condensation; cell differentiation; ossification; regulation of bone mineralization

Disease: Keutel Syndrome