Dynamin-like GTPase MGM1, mitochondrial (MGM1) Recombinant Protein | MGM1 recombinant protein

Recombinant Saccharomyces cerevisiae Dynamin-like GTPase MGM1, mitochondrial (MGM1)

• Gene Names: MGM1; MNA1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Dynamin-like GTPase MGM1; mitochondrial (MGM1); Recombinant Saccharomyces cerevisiae Dynamin-like GTPase MGM1; Recombinant Dynamin-like GTPase MGM1; mitochondrial; Mitochondrial division and morphology protein 17 Mitochondrial genome maintenance protein 1 Cleaved into the following 2 chains: 1. Dynamin-like GTPase MG; MGM1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 140-881
• Sequence: ATLIAATSLDDDESKRQGDPKDDDDEDDDDEDDENDSVDTTQDEMLNLTKQMIEIRTILNKVDSSSAHLTLPSIVVIGSQSSGKSSVLESIVGREFLPKGSNMVTRRPIELTLVNTPNSNNVTADFPSMRLYNIKDFKEVKRMLMELNMAVPTSEAVSEEPIQLTIKSSRVPDLSLVDLPGYIQVEAADQPIELKTKIRDLCEKYLTAPNIILAISAADVDLANSSALKASKAADPKGLRTIGVITKLDLVDPEKARSILNNKKYPLSMGYVGVITKTPSSINRKHLGLFGEAPSSSLSGIFSKGQHGQSSGEENTNGLKQIVSHQFEKAYFKENKKYFTNCQVSTKKLREKLIKILEISMSNALEPTSTLIQQELDDTSYLFKVEFNDRHLTPKSYLLNNIDVLKLGIKEFQEKFHRNELKSILRAELDQKVLDVLATRYWKDDNLQDLSSSKLESDTDMLYWHKKLELASSGLTKMGIGRLSTMLTTNAILKELDNILESTQLKNHELIKDLVSNTAINVLNSKYYSTADQVENCIKPFKYEIDLEERDWSLARQHSINLIKEELRQCNSRYQAIKNAVGSKKLANVMGYLENESNLQKETLGMSKLLLERGSEAIFLDKRCKVLSFRLKMLKNKCHSTIEKDRCPEVFLSAVSDKLTSTAVLFLNVELLSDFFYNFPIELDRRLTLLGDEQVEMFAKEDPKISRHIELQKRKELLELALEKIDSILVFKKSYKGVSKNL
• Sequence Length: 881

• Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 116006501
• NCBI GeneID: 854386
• NCBI Accession #: NP_014854.2
• NCBI GenBank Nucleotide #: NM_001183630.1
• UniProt Accession #: P32266; Q02609; Q08627; D6W2R7
• Molecular Weight: 99,178 Da
• NCBI Official Full Name: Mgm1p
• NCBI Official Symbol: MGM1
• NCBI Official Synonym Symbols: MNA1
• NCBI Protein Information: Mgm1p
• UniProt Protein Name: Dynamin-like GTPase MGM1, mitochondrial
• Protein Family: Dynamin-like GTPase
• UniProt Gene Name: MGM1
• UniProt Synonym Gene Names: MDM17; l-MGM1; s-MGM1
• UniProt Entry Name: MGM1_YEAST

Uniprot Description

Function: Dynamin-related GTPase required for mitochondrial fusion. Coordinates interaction between the inner and outer mitochondrial membrane to promote the formation of the double membrane. Ref.7 Ref.8 Ref.9 Ref.11

Subunit structure: Homooligomer. Associates with FZO1 through interaction with the intermembrane space domain of UGO1 which binds FZO1 through its cytoplasmic domain. Ref.8 Ref.9 Ref.13

Subcellular location: Dynamin-like GTPase MGM1 large isoform: Mitochondrion inner membrane; Single-pass type II membrane protein; Intermembrane side Ref.7 Ref.9 Ref.12. Dynamin-like GTPase MGM1 small isoform: Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side Ref.7 Ref.9 Ref.12.

Post-translational modification: Cleavage of the transit peptide by mitochondrial processing protease (MPP) produces a long integral membrane form of MGM1 (l-MGM1). Further processing by the rhomboid protease PCP1 produces a short peripheral membrane form of MGM1 (s-MGM1). Both isoforms are required for full activity.

Miscellaneous: Deletion of MGM1 causes the mitochondria to fragment and aggregate, and subsequently to lose their mitochondrial DNA and become respiration deficient.

Sequence similarities: Belongs to the dynamin family.Contains 1 GED domain.

Sequence caution: The sequence CAA44637.1 differs from that shown. Reason: Frameshift at position 4. The sequence CAA63174.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA99426.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA99428.1 differs from that shown. Reason: Erroneous initiation.