Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitoferrin-1 (SLC25A37) Standard Curve.)
Human Mitoferrin-1 (SLC25A37) ELISA Kit | MFRN elisa kit
Human Mitoferrin-1 (SLC25A37) ELISA Kit
Gene Names
SLC25A37; MSC; MFRN; MSCP; HT015; MFRN1; PRO1278; PRO1584; PRO2217
Reactivity
Human
Synonyms
Mitoferrin-1 (SLC25A37); Human Mitoferrin-1 (SLC25A37) ELISA Kit; SLC25A37; HT015; MSC; MSCP; PRO1278; PRO1584; PRO2217; mitochondrial solute carrier protein; mitoferrin; predicted protein of HQ2217; MFRN elisa kit
Reactivity
Human
Specificity
Human Mitoferrin-1 (SLC25A37) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC25A37. No significant cross-reactivity or interference between Human SLC25A37 and analogues was observed.
Sequence Length
338
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitoferrin-1 (SLC25A37) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitoferrin-1 (SLC25A37) Standard Curve.)
Related Product Information for MFRN elisa kit
Description: This Human Mitoferrin-1 (SLC25A37) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC25A37 in samples. An antibody specific for SLC25A37 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC25A37 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC25A37 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC25A37 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SLC25A37 belongs to the SLC25 family of mitochondrial carrier proteins.Shaw et al. (2006) cloned mouse Slc25a37, which they called mitoferrin. Northern blot analysis detected expression in all tissues examined, with highest levels in hematopoietic organs, fetal liver, bone marrow, and spleen. In developing mouse embryos, mitoferrin was first detected at embryonic day 7.5 in the extraembryonic yolk sac, coincident with the appearance of blood islands. By searching databases for SLC25 family members, Haitina et al. (2006) identified 2 splice variants of SLC25A37. The deduced protein contains 351 amino acids.SLC25A37 gene maps to chromosome 8p21. The mouse Slc25a37 gene maps to chromosome 14.
Background: SLC25A37 belongs to the SLC25 family of mitochondrial carrier proteins.Shaw et al. (2006) cloned mouse Slc25a37, which they called mitoferrin. Northern blot analysis detected expression in all tissues examined, with highest levels in hematopoietic organs, fetal liver, bone marrow, and spleen. In developing mouse embryos, mitoferrin was first detected at embryonic day 7.5 in the extraembryonic yolk sac, coincident with the appearance of blood islands. By searching databases for SLC25 family members, Haitina et al. (2006) identified 2 splice variants of SLC25A37. The deduced protein contains 351 amino acids.SLC25A37 gene maps to chromosome 8p21. The mouse Slc25a37 gene maps to chromosome 14.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mitoferrin-1 isoform 1
NCBI Official Synonym Full Names
solute carrier family 25 member 37
NCBI Official Symbol
SLC25A37
NCBI Official Synonym Symbols
MSC; MFRN; MSCP; HT015; MFRN1; PRO1278; PRO1584; PRO2217
NCBI Protein Information
mitoferrin-1
UniProt Protein Name
Mitoferrin-1
UniProt Gene Name
SLC25A37
UniProt Synonym Gene Names
MFRN; MSCP
UniProt Entry Name
MFRN1_HUMAN
NCBI Description
SLC25A37 is a solute carrier localized in the mitochondrial inner membrane. It functions as an essential iron importer for the synthesis of mitochondrial heme and iron-sulfur clusters (summary by Chen et al., 2009 [PubMed 19805291]).[supplied by OMIM, Jan 2011]
Uniprot Description
SLC25A37: Mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells, thereby playing an essential role in heme biosynthesis. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme. Belongs to the mitochondrial carrier family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Mitochondrial
Chromosomal Location of Human Ortholog: 8p21.2
Cellular Component: integral to membrane; mitochondrial inner membrane
Molecular Function: iron ion transmembrane transporter activity; structural constituent of ribosome
Biological Process: iron ion homeostasis; mitochondrial iron ion transport; translation
Research Articles on MFRN
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Product Notes
The Human MFRN slc25a37 (Catalog #AAA9715991) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715991 ELISA Kit recognizes Human MFRN. It is sometimes possible for the material contained within the vial of "Mitoferrin-1 (SLC25A37), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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