Rabbit anti-Human SLC25A37 Polyclonal Antibody | anti-SLC25A37 antibody

SLC25A37, CT (SLC25A37, MFRN, MSCP, Mitoferrin-1, Mitochondrial iron transporter 1, Mitochondrial solute carrier protein, Solute carrier family 25 member 37) (FITC)

Cat. Num. AAA6348267

• Gene Names: SLC25A37; MSC; MFRN; MSCP; HT015; MFRN1; PRO1278; PRO1584; PRO2217
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SLC25A37; Polyclonal Antibody; CT (SLC25A37; MFRN; MSCP; Mitoferrin-1; Mitochondrial iron transporter 1; Mitochondrial solute carrier protein; Solute carrier family 25 member 37) (FITC); mitochondrial iron transporter 1; mitochondrial solute carrier protein; Solute carrier family 25 member 37; anti-SLC25A37 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SLC25A37 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SLC25A37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309~338 amino acids from the C-terminal region of human SLC25A37.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SLC25A37 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). SLC25A37 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(SLC25A37 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC25A37 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SLC25A37 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SLC25A37 antibody

SLC25A37 belongs to the SLC25 family of mitochondrial carrier proteins (Haitina et al., 2006 [PubMed 16949250]).[supplied by OMIM].

Product Categories/Family for anti-SLC25A37 antibody

Antibodies; Mitochondrial Proteins

References

Kaneko, K., et al. FEBS J. 276(5):1370-1382(2009). Haitina, T., et al. Genomics 88(6):779-790(2006). Shaw, G.C., et al. Nature 440(7080):96-100(2006). Li, Q.Z., et al. Mamm. Genome 12(11):830-836(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 82775373
• NCBI GeneID: 51312
• NCBI Accession #: NP_057696.2
• NCBI GenBank Nucleotide #: NM_016612.3
• UniProt Accession #: Q9NYZ2; Q53FT7; Q69YJ8; Q969S1; Q9P0J2; A2RU93
• Molecular Weight: 21,125 Da
• NCBI Official Full Name: mitoferrin-1 isoform 1
• NCBI Official Synonym Full Names: solute carrier family 25 (mitochondrial iron transporter), member 37
• NCBI Official Symbol: SLC25A37
• NCBI Official Synonym Symbols: MSC; MFRN; MSCP; HT015; MFRN1; PRO1278; PRO1584; PRO2217
• NCBI Protein Information: mitoferrin-1
• UniProt Protein Name: Mitoferrin-1
• Protein Family: Mitoferrin
• UniProt Gene Name: SLC25A37
• UniProt Synonym Gene Names: MFRN; MSCP
• UniProt Entry Name: MFRN1_HUMAN

NCBI Description

SLC25A37 is a solute carrier localized in the mitochondrial inner membrane. It functions as an essential iron importer for the synthesis of mitochondrial heme and iron-sulfur clusters (summary by Chen et al., 2009 [PubMed 19805291]).[supplied by OMIM, Jan 2011]

Uniprot Description

SLC25A37: Mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells, thereby playing an essential role in heme biosynthesis. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme. Belongs to the mitochondrial carrier family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: integral to membrane; mitochondrial inner membrane

Molecular Function: iron ion transmembrane transporter activity; structural constituent of ribosome

Biological Process: iron ion homeostasis; mitochondrial iron ion transport; translation