METAP1 recombinant protein

METAP1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120133

• Gene Names: METAP1; MAP1A; MetAP1A
• Purity: >95% as determined by SDS-PAGE
• Synonyms: METAP1; METAP1 Protein; Human; Recombinant (His Tag); Human METAP1 Protein (His Tag); MAP1A Protein; MetAP1A Protein; methionyl aminopeptidase 1; METAP1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: His52-Phe386

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human METAP1 (NP_055958.2)(His52-Phe386) was expressed with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for METAP1 recombinant protein

Background: Processing of the N-terminal initiator methionine or formylated methionine is an essential cellular process conserved from prokaryotes to eukaryotes. The proteolytic removal of N-terminal methionine from nascent peptides is catalyzed by a family of enzymes known as methionine aminopeptidases (MetAPs) and is essential for cell growth. METAP1 and METAP2 have different substrate specificity due to the differences in both size and shape of the active sites. As a member of the M24 family of metalloproteases, METAP1 plays an important role in G(2)/M phase regulation of the cell cycle and may serve as a promising target for the discovery and development of new anticancer agents.

References

1. Lowther, W.T. and B.W. Matthews, 2000, Biochim. Biophys. Acta. 1477: 157-167. 2. Addlaqatta, A. et al., 2005, Biochemistry. 44: 14741-14749. 3. Hu, X. et al., 2006, Proc. Natl. Acad. Sci. U.S.A. 103:18148-18153.

NCBI and Uniprot Product Information

• NCBI GI #: 164420681
• NCBI GeneID: 23173
• NCBI Accession #: NP_055958.2
• NCBI GenBank Nucleotide #: NM_015143.2
• UniProt Accession #: P53582; B4E2E6
• Molecular Weight: 43,215 Da
• NCBI Official Full Name: methionine aminopeptidase 1
• NCBI Official Synonym Full Names: methionyl aminopeptidase 1
• NCBI Official Symbol: METAP1
• NCBI Official Synonym Symbols: MAP1A; MetAP1A
• NCBI Protein Information: methionine aminopeptidase 1; MAP 1; metAP 1; peptidase M 1
• UniProt Protein Name: Methionine aminopeptidase 1
• Protein Family: Methionine aminopeptidase
• UniProt Gene Name: METAP1
• UniProt Entry Name: MAP11_HUMAN

Uniprot Description

METAP1: a peptidase that removes the amino-terminal methionine from nascent proteins.

Protein type: EC 3.4.11.18; Protease

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: metalloexopeptidase activity; metal ion binding; aminopeptidase activity

Biological Process: rhodopsin mediated signaling; regulation of translation; phototransduction, visible light; regulation of rhodopsin mediated signaling; peptidyl-methionine modification; proteolysis; N-terminal protein amino acid modification