Western Blot (WB)
(METAP1 Antibody western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the METAP1 antibody detected the METAP1 protein (arrow).)
Mouse anti-Human METAP1 Monoclonal Antibody | anti-METAP1 antibody
METAP1 (Methionine Aminopeptidase 1, MetAP 1, MAP 1, Peptidase M 1, KIAA0094)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100~250
Western Blot (WB)
(METAP1 Antibody western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the METAP1 antibody detected the METAP1 protein (arrow).)
Western Blot (WB)
(METAP1 Antibody western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the METAP1 antibody detected the METAP1 protein (arrow).)
NCBI and Uniprot Product Information
Uniprot Description
Function: Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Required for normal progression through the cell cycle. Ref.7 Ref.9
Catalytic activity: Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides. Ref.9
Cofactor: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site
By similarity. Ref.7Binds 1 sodium ion per subunit. The sodium ion has a structural role. Ref.7
Subunit structure: Associates with the 60S ribosomal subunit of the 80S translational complex
By similarity.
Subcellular location: Cytoplasm
By similarity HAMAP-Rule MF_03174.
Sequence similarities: Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.
Sequence caution: The sequence AAH30054.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA07679.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on METAP1
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Product Notes
The METAP1 metap1 (Catalog #AAA646402) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The METAP1 (Methionine Aminopeptidase 1, MetAP 1, MAP 1, Peptidase M 1, KIAA0094) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's METAP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA. Dilution: ELISA: 1:1,000 Western Blot: 1:100~250. Researchers should empirically determine the suitability of the METAP1 metap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "METAP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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