Midasin (MDN1) Recombinant Protein | MDN1 recombinant protein

Recombinant Human Midasin (MDN1) , partial

• Gene Names: MDN1; Rea1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Midasin (MDN1); Recombinant Human Midasin (MDN1); partial; MDN1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 5,596

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 24415404
• NCBI GeneID: 23195
• NCBI Accession #: NP_055426.1
• NCBI GenBank Nucleotide #: NM_014611.2
• UniProt Accession #: Q9NU22; O15019; Q5T794
• Molecular Weight: 632,820 Da
• NCBI Official Full Name: midasin
• NCBI Official Synonym Full Names: midasin AAA ATPase 1
• NCBI Official Symbol: MDN1
• NCBI Official Synonym Symbols: Rea1
• NCBI Protein Information: midasin
• UniProt Protein Name: Midasin
• Protein Family: Midasin
• UniProt Gene Name: MDN1
• UniProt Synonym Gene Names: KIAA0301

Uniprot Description

Nuclear chaperone required for maturation and nuclear export of pre-60S ribosome subunits (PubMed:27814492). Functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus (). At an early stage in 60S maturation, mediates the dissociation of the PeBoW complex (PES1-BOP1-WDR12) from early pre-60S particles, rendering them competent for export from the nucleolus to the nucleoplasm (). Subsequently recruited to the nucleoplasmic particles through interaction with SUMO-conjugated PELP1 complex (PubMed:27814492). This binding is only possible if the 5S RNP at the central protuberance has undergone the rotation to complete its maturation ().