Immunohistochemistry (IHC)
(MBS9600222 at 1/100 staining human testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit Tubulin gamma Polyclonal Antibody | anti-TUBG1 antibody
Tubulin gamma Antibody
Gene Names
TUBG1; TUBG; GCP-1; CDCBM4; TUBGCP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Tubulin gamma; Polyclonal Antibody; Tubulin gamma Antibody; Gamma 1 tubulin; Gamma 2 tubulin; Gamma Tubulin 1; Gamma Tubulin 2; Gamma tubulin complex component 1; Gamma-2-tubulin; GCP 1; GCP1; MGC131994; TBG2_HUMAN; TUBG; TUBG1; TUBG2; TUBGCP1; Tubulin gamma 1 chain; Tubulin gamma 2 chain; Tubulin gamma complex-associated protein 1; Tubulin gamma-2 chain; tubulin; gamma 1; gamma 2; gamma polypeptide; Xgam; anti-TUBG1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Tubulin gamma antibody detects endogenous levels of total Tubulin gamma
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
451
Applicable Applications for anti-TUBG1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:3000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human Tubulin gamma
Subcellular Location
Cytoplasm > Cytoskeleton > Centrosome.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9600222 at 1/100 staining human testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9600222 at 1/100 staining human testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis on mouse brain cell lysate using Tubulin gamma Antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis on mouse brain cell lysate using Tubulin gamma Antibody.The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-TUBG1 antibody
Function: Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
Subunit Structure: Interacts with TUBGCP2 and TUBGCP3 (PubMed:9566969, PubMed:9566969). Interacts with B9D2 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2 (PubMed:17959831). Interacts with PIFO (PubMed:20643351). Interacts with SAS6 and NUP62 at the centrosome (PubMed:24107630).
Post-translational Modifications: Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Similarity: Belongs to the tubulin family.
Subunit Structure: Interacts with TUBGCP2 and TUBGCP3 (PubMed:9566969, PubMed:9566969). Interacts with B9D2 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2 (PubMed:17959831). Interacts with PIFO (PubMed:20643351). Interacts with SAS6 and NUP62 at the centrosome (PubMed:24107630).
Post-translational Modifications: Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Similarity: Belongs to the tubulin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 51 kDa
Predicted: 52 kDa
Predicted: 52 kDa
NCBI Official Full Name
tubulin gamma-1 chain
NCBI Official Synonym Full Names
tubulin gamma 1
NCBI Official Symbol
TUBG1
NCBI Official Synonym Symbols
TUBG; GCP-1; CDCBM4; TUBGCP1
NCBI Protein Information
tubulin gamma-1 chain
UniProt Protein Name
Tubulin gamma-1 chain
Protein Family
UniProt Gene Name
TUBG1
UniProt Synonym Gene Names
TUBG; GCP-1
NCBI Description
This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]
Uniprot Description
Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
Research Articles on TUBG1
Similar Products
Product Notes
The TUBG1 tubg1 (Catalog #AAA9600222) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Tubulin gamma Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Tubulin gamma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:3000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the TUBG1 tubg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tubulin gamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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