Mouse midkine (neurite growth-promoting factor 2) ELISA Kit | MDK elisa kit

Mouse Midkine, MDK ELISA Kit

Cat. Num. AAA905741

• Gene Names: Mdk; MK; Mek
• Reactivity: Mouse
• Synonyms: midkine (neurite growth-promoting factor 2); Mouse Midkine; MDK ELISA Kit; Mouse Midkine (MDK) ELISA kit; FLJ27379; MK; NEGF2; OTTHUMP00000197041; OTTHUMP00000197042; OTTHUMP00000197045; midkine; MDK elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse MDK. No significant cross-reactivity or interference between mouse MDK and analogues was observed.
• Sequence Length: 140

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: <7.81 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for MDK elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MDK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MDK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDK bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 59894790
• NCBI GeneID: 17242
• NCBI Accession #: NP_001012335.1
• NCBI GenBank Nucleotide #: NM_001012335.2
• UniProt Accession #: P12025
• Molecular Weight: 15,434 Da
• NCBI Official Full Name: midkine isoform a
• NCBI Official Synonym Full Names: midkine
• NCBI Official Symbol: Mdk
• NCBI Official Synonym Symbols: MK; Mek
• NCBI Protein Information: midkine; retinoic acid-responsive protein; retinoic acid-induced differentiation factor
• UniProt Protein Name: Midkine
• Protein Family: Midkine
• UniProt Gene Name: Mdk
• UniProt Synonym Gene Names: Mk; MK
• UniProt Entry Name: MK_MOUSE

NCBI Description

This gene encodes a secreted growth factor that belongs to the pleiotrophin/midkine heparin-binding protein family and functions in a variety of biological processes. The encoded cytokine promotes the growth, differentiation, survival and migration of several target cells including leucocytes involved in inflammation. This protein plays a role in the formation of scar tissue and intraperitoneal adhesions, and promotes neurite outgrowth and neuron survival. The protein encoded by this gene is associated with obesity and inhibition of insulin signaling in fat cells. A pseudogene of this gene is present on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

MDK: Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. Belongs to the pleiotrophin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: cell projection; cytoplasm; extracellular region

Molecular Function: heparin binding; growth factor activity

Biological Process: cell migration; behavioral fear response; short-term memory; cerebellar granular layer development; adrenal gland development; multicellular organismal development; positive regulation of transcription, DNA-dependent; positive regulation of cell division; hippocampus development; response to wounding; negative regulation of neuron apoptosis; cell differentiation; defecation; regulation of behavior