SDS-PAGE
Malate dehydrogenase Recombinant Protein | mdh recombinant protein
Recombinant E Coli Malate dehydrogenase
Gene Names
mdh; ECK3225; JW3205
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Malate dehydrogenase; Recombinant E Coli Malate dehydrogenase; mdh recombinant protein
Host
E Coli
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Liquid containing glycerol
Sequence Positions
1-312aa; Full Length
Sequence
MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAVDLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLVQQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAELKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVEERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK
Sequence Length
312
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for mdh recombinant protein
Catalyzes the reversible oxidation of malate to oxaloacetate.
References
Complete nucleotide sequence of the Escherichia coli gene encoding malate dehydrogenase.McAlister-Henn L., Blaber M., Bradshaw R.A., Nisco S.J.Nucleic Acids Res. 15:4993-4993(1987)
Cloning and sequence of the mdh structural gene of Escherichia coli coding for malate dehydrogenase.Vogel R.F., Entian K.-D., Mecke D.Arch. Microbiol. 149:36-42(1987)
The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997)
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006)
Isolation and expression of the Escherichia coli gene encoding malate dehydrogenase.Sutherland P., McAlister-Henn L.J. Bacteriol. 163:1074-1079(1985)
Malate dehydrogenase
isolation from E. coli and comparison with the eukaryotic mitochondrial and cytoplasmic forms.Fernley R.T., Lentz S.R., Bradshaw R.A.Biosci. Rep. 1:497-507(1981)
Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.Link A.J., Robison K., Church G.M.Electrophoresis 18:1259-1313(1997)
Structural studies of malate dehydrogenases (MDHs)
MDHs in Brevundimonas species are the first reported MDHs in Proteobacteria which resemble lactate dehydrogenases in primary structure.Charnock C.J. Bacteriol. 179:4066-4070(1997)
Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases.Henzel W.J., Billeci T.M., Stults J.T., Wong S.C., Grimley C., Watanabe C.Proc. Natl. Acad. Sci. U.S.A. 90:5011-5015(1993)
Bacterial defense against aging
role of the Escherichia coli ArcA regulator in gene expression, readjusted energy flux and survival during stasis.Nystroem T., Larsson C., Gustafsson L.EMBO J. 15:3219-3228(1996)
Frutiger S., Hughes G.J., Pasquali C., Hochstrasser D.F.Submitted (FEB-1996)
to UniProtKB
Protein identification with N and C-terminal sequence tags in proteome projects.Wilkins M.R., Gasteiger E., Tonella L., Ou K., Tyler M., Sanchez J.-C., Gooley A.A., Walsh B.J., Bairoch A., Appel R.D., Williams K.L., Hochstrasser D.F.J. Mol. Biol. 278:599-608(1998)
Molecular genetic basis of allelic polymorphism in malate dehydrogenase (mdh)
in natural populations of Escherichia coli and Salmonella enterica.Boyd E.F., Nelson K., Wang F.-S., Whittam T.S., Selander R.K.Proc. Natl. Acad. Sci. U.S.A. 91:1280-1284(1994)
Evolutionary relationships among pathogenic and nonpathogenic Escherichia coli strains inferred from multilocus enzyme electrophoresis and mdh sequence studies.Pupo G.M., Karaolis D.K., Lan R., Reeves P.R.Infect. Immun. 65:2685-2692(1997)
Population genetics of Escherichia coli in a natural population of native Australian rats.Pupo G.M., Lan R., Reeves P.R., Baverstock P.R.Environ. Microbiol. 2:594-610(2000)
Escherichia coli proteome analysis using the gene-protein database.VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.Electrophoresis 18:1243-1251(1997)
Crystal structure of Escherichia coli malate dehydrogenase. A complex of the apoenzyme and citrate at 1.87-A resolution.Hall M.D., Levitt D.G., Banaszak L.J.J. Mol. Biol. 226:867-882(1992)
Crystal structure of a ternary complex of Escherichia coli malate dehydrogenase citrate and NAD at 1.9 A resolution.Hall M.D., Banaszak L.J.J. Mol. Biol. 232:213-222(1993)
Structural analyses of a malate dehydrogenase with a variable active site.Bell J.K., Yennawar H.P., Wright S.K., Thompson J.R., Viola R.E., Banaszak L.J.J. Biol. Chem. 276:31156-31162(2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32.3kD
NCBI Official Full Name
malate dehydrogenase, NAD(P)-binding
NCBI Official Symbol
mdh
NCBI Official Synonym Symbols
ECK3225; JW3205
NCBI Protein Information
malate dehydrogenase, NAD(P)-binding
UniProt Protein Name
Malate dehydrogenase
Protein Family
UniProt Gene Name
mdh
UniProt Entry Name
MDH_ECOLI
NCBI Description
Mdh is elevated 4X during growth at 4C (enabled by Oleispira antarctica Cpn10/60 transgene). [More information is available at EcoGene: EG10576]. Malate dehydrogenase (Mdh) catalyzes the reversible oxidation of malate to generate oxaloacetate. [More information is available at EcoCyc: EG10576].
Uniprot Description
Catalyzes the reversible oxidation of malate to oxaloacetate.
Research Articles on mdh
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Product Notes
The mdh mdh (Catalog #AAA1265486) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-312aa; Full Length. The amino acid sequence is listed below: MKVAVLGAAG GIGQALALLL KTQLPSGSEL SLYDIAPVTP GVAVDLSHIP TAVKIKGFSG EDATPALEGA DVVLISAGVA RKPGMDRSDL FNVNAGIVKN LVQQVAKTCP KACIGIITNP VNTTVAIAAE VLKKAGVYDK NKLFGVTTLD IIRSNTFVAE LKGKQPGEVE VPVIGGHSGV TILPLLSQVP GVSFTEQEVA DLTKRIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR ALQGEQGVVE CAYVEGDGQY ARFFSQPLLL GKNGVEERKS IGTLSAFEQN ALEGMLDTLK KDIALGEEFV NK. It is sometimes possible for the material contained within the vial of "Malate dehydrogenase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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