Horse Monocyte Chemotactic Protein 3 ELISA Kit | MCP-3 elisa kit

Horse Monocyte Chemotactic Protein 3 ELISA Kit

Cat. Num. AAA29950

• Reactivity: Horse
• Synonyms: Monocyte Chemotactic Protein 3; Horse Monocyte Chemotactic Protein 3 ELISA Kit; MCP-3 elisa kit

• Reactivity: Horse
• Specificity: No significant cross-reactivity or interference between Horse MCP-3 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 6.25 pg/ml - 200 pg/ml.
• Sensitivity: 1.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated MCP-3 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse MCP-3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MCP-3 and MCP-3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MCP-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for MCP-3 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated MCP-3 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse MCP-3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MCP-3 and MCP-3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MCP-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 313708
• UniProt Accession #: P80098; Q569J6
• Molecular Weight: 11,200 Da
• NCBI Official Full Name: monocyte chemotactic protein-3
• UniProt Protein Name: C-C motif chemokine 7
• UniProt Gene Name: CCL7
• UniProt Synonym Gene Names: MCP3; SCYA6; SCYA7; MCP-3
• UniProt Entry Name: CCL7_HUMAN

Uniprot Description

CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CCR1 chemokine receptor binding; chemokine activity; CCR2 chemokine receptor binding

Biological Process: cellular calcium ion homeostasis; monocyte chemotaxis; regulation of cell shape; cell-cell signaling; eosinophil chemotaxis; response to gamma radiation; immune response; cytoskeleton organization and biogenesis; inflammatory response; chemotaxis; signal transduction; positive regulation of cell migration