MCM2 / DNA Replication Licensing Factor MCM2 Recombinant Protein | MCM2 recombinant protein

Human MCM2 / DNA Replication Licensing Factor MCM2 Recombinant Protein

• Gene Names: MCM2; BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: MCM2 / DNA Replication Licensing Factor MCM2; Human MCM2 / DNA Replication Licensing Factor MCM2 Recombinant Protein; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28; BM28; CCNL1; CDCL1; KIAA0030; MCM2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 904

• Applicable Applications for MCM2 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with DBF4 (By similarity). Interacts with KAT7. May interact with MCM10.
• Entry Function: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 33356547
• NCBI GeneID: 4171
• NCBI Accession #: NP_004517.2
• NCBI GenBank Nucleotide #: NP_004517.2
• UniProt Accession #: P49736
• NCBI Official Full Name: DNA replication licensing factor MCM2
• NCBI Official Synonym Full Names: minichromosome maintenance complex component 2
• NCBI Official Symbol: MCM2
• NCBI Official Synonym Symbols: BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN
• NCBI Protein Information: DNA replication licensing factor MCM2
• UniProt Protein Name: DNA replication licensing factor MCM2
• Protein Family: DNA replication licensing factor
• UniProt Gene Name: MCM2
• UniProt Synonym Gene Names: BM28; CCNL1; CDCL1; KIAA0030
• UniProt Entry Name: MCM2_HUMAN

NCBI Description

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

Uniprot Description

MCM2: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.

Protein type: DNA-binding; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: microtubule cytoskeleton; nucleoplasm; MCM complex; cytoplasm; chromatin; nuclear origin of replication recognition complex; nucleus

Molecular Function: protein binding; DNA helicase activity; DNA binding; histone binding; metal ion binding; DNA replication origin binding; ATP binding

Biological Process: DNA unwinding during replication; nucleosome assembly; DNA replication initiation; DNA strand elongation during DNA replication; mitotic cell cycle; cell cycle; DNA replication; G1/S transition of mitotic cell cycle