Cat Induced myeloid leukemia cell differentiation protein Mcl-1 ELISA Kit | MCL1 elisa kit
Cat Induced myeloid leukemia cell differentiation protein Mcl-1 (MCL1) ELISA Kit
Gene Names
MCL1; mcl-1
Reactivity
Cat
Synonyms
Induced myeloid leukemia cell differentiation protein Mcl-1; Cat Induced myeloid leukemia cell differentiation protein Mcl-1 (MCL1) ELISA Kit; BCL2L3; EAT; MCL1L; MCL1S; MGC104264; MGC1839; Mcl-1; TM; induced myeloid leukemia cell differentiation protein Mcl-1|myeloid cell leukemia ES|myeloid cell leukemia sequence 1|myeloid cell leukemia; MCL1 elisa kit
Reactivity
Cat
Specificity
This assay has high sensitivity and excellent specificity for detection of Cat MCL1. No significant cross-reactivity or interference between Cat MCL1 and analogues was observed.
Sequence Length
197
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for MCL1 elisa kit
Induced myeloid leukemia cell differentiation protein Mcl-1 is a protein belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MCL1 in samples. An antibody specific for MCL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MCL1 in samples. An antibody specific for MCL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,635 Da
NCBI Official Full Name
induced myeloid leukemia cell differentiation protein Mcl-1 homolog
NCBI Official Symbol
MCL1
NCBI Official Synonym Symbols
mcl-1
NCBI Protein Information
induced myeloid leukemia cell differentiation protein Mcl-1 homolog
UniProt Protein Name
Induced myeloid leukemia cell differentiation protein Mcl-1 homolog
Protein Family
UniProt Gene Name
MCL1
Uniprot Description
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis ().
Similar Products
Product Notes
The Cat MCL1 mcl1 (Catalog #AAA284765) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA284765 ELISA Kit recognizes Cat MCL1. It is sometimes possible for the material contained within the vial of "Induced myeloid leukemia cell differentiation protein Mcl-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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