Related Product Information for Mbl2 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Mbl2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Mbl2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Mbl2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Mbl2 can be calculated.
NCBI Description
This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]
Uniprot Description
MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 10q11.2
Cellular Component: cell surface; collagen; extracellular region; extracellular space
Molecular Function: calcium ion binding; calcium-dependent protein binding; mannose binding; protein binding; receptor binding
Biological Process: acute-phase response; complement activation; complement activation, classical pathway; complement activation, lectin pathway; defense response to bacterium; defense response to Gram-positive bacterium; innate immune response; killing by host of symbiont cells; negative regulation of viral reproduction; opsonization; positive regulation of phagocytosis; response to oxidative stress
Disease: Mannose-binding Protein Deficiency
Product Notes
The Mouse Mbl2 mbl2 (Catalog #
AAA764842) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA764842 ELISA Kit recognizes Mouse Mbl2.
It is sometimes possible for the material contained within the vial of
"Mannose-binding protein C, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
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to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
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