Fibroblast growth factor 21 Active Protein

Fibroblast growth factor 21, Recombinant, Human (FGF-21)

Cat. Num. AAA651071

• Purity: Purified; > 97%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: < 1.0 EU per 1ug of the protein as determined by the LAL method.
• Synonyms: Fibroblast growth factor 21; Recombinant; Human (FGF-21); Fibroblast growth factor 21 active protein

• Host: E Coli
• Purity/Purification: Purified; > 97%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: < 1.0 EU per 1ug of the protein as determined by the LAL method.
• Form/Format: Supplied as a lyophilized powder in 10 mM MES, 50 mM sodium sulfate, 0.2 mM EDTA and

• Biological Activity: Measured by its ability to stimulate the proliferation of NIH3T3 cells. The ED 50 for this effect is typically 0.12-0.6ug/ml in the presence of 5ug/ml of rmKlotho-beta; Measured in a cell proliferation assay using human FGF RIIIc transfected BaF3 mouse pro­B cells. The ED50 for this effect is typically 0.06 ­ 0.4ug/ml in the presence of rmKlotho-beta; ; Storage and Stability:; Lyophilized powder stored at -20 degree C to -70 degree C is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Product reconstituted under sterile conditions is stable for 1 month at 4 degree C and for 3 months at --20 degree C to -70 degree C. Avoid freeze-thaw cycles.
• Endotoxin Level: 1 EU/ug of the protein as determined by the LAL method.
• Preparation and Storage: -20 degree C/-70 degree C

SDS-Page

(1ug/lane of Recombinant Human FGF-21 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 24kD.)

Related Product Information for Fibroblast growth factor 21 active protein

Human FGF-21 Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family, which currently contains 22 human members. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a b-trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members apparently exhibit poor binding to ECM, resulting in highly diffusible molecules (3). The c-DNA for FGF-21 predicts a 209 aa polypeptide that contains a 28 aa signal sequence and a 181 aa mature region (4). Notably, FGF-21, as well as FGF-19 shows limited binding to heparin (4). One potential alternate splice form has been reported. It shows a 43 aa substitution for the C-terminal 12 aa of the standard form (5). Mature human FGF-21 shows 81% aa identity to mouse FGF-21, and is known to be active on mouse cells (4, 6). The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism, all three are induced by a nuclear receptor heterodimer that includes RXR, and all three utilize Klotho family members for signal transduction (7, 8, 9). FGF-21 is produced by hepatocytes in response to free fatty acid (FFA) stimulation of a PPARa/RXR dimeric complex (3, 7, 10, 11). This situation occurs clinically during starvation, or following the ingestion of a high-fat/low-carbohydrate diet. Upon FGF-21 secretion, white adipose tissue is induced to release FFAs from triglyceride stores. Once FFAs reach hepatocytes, they are oxidized and reduced to acetyl-CoA. The acetyl-CoA is recombined into 4-carbon ketone bodies (acetoacetate and b-hydroxybutyrate), released, and transported to peripheral tissues for TCA processing and energy generation (11, 12).

Product Categories/Family for Fibroblast growth factor 21 active protein

Growth Factors; Cytokines; Growth Factors-FGF

NCBI and Uniprot Product Information

• NCBI GI #: 114731554
• Molecular Weight: Based on N-terminal amino acid sequencing, the recombinant human FGF-21 starts at the histidine tag and has a predicted molecular mass of approximately 20.2kD.
• NCBI Official Full Name: fibroblast growth factor 21
• Protein Family: Fibroblast growth factor

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular region

Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding

Biological Process: positive regulation of glucose import; cell-cell signaling; positive regulation of cell proliferation; positive regulation of low-density lipoprotein receptor biosynthetic process; signal transduction