Human Matrilin-3 ELISA Kit | MATN3 elisa kit

Human Matrilin-3 PicoKine ELISA Kit

Cat. Num. AAA1752629

• Gene Names: MATN3; HOA; OS2; EDM5; DIPOA; OADIP
• Reactivity: Human
• Synonyms: Matrilin-3; Human Matrilin-3 PicoKine ELISA Kit; Human Matrilin-3; matrilin 3; MATN3; MATN3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human MATN3
• Sequence Length: 486

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Detection Range: 93.7pg/ml-6000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MATN3 elisa kit

Principle of the Assay: The Picokine™ Human MATN3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MATN3 with a 96-well strip plate that is pre-coated with antibody specific for MATN3. The detection antibody is a biotinylated antibody specific for MATN3. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human MATN3 with immunogen: Expression system for standard: NSO, Immunogen sequence: D29-R486. The kit is analytically validated with ready to use reagents. To measure Human MATN3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human MATN3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human MATN3 in the sample.!!Background/Introduction: Matrilin-3 is a protein that in humans is encoded by the MATN3 gene. This gene is mapped to 2p24.1. This gene encodes a member of von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains two von Willebrand factor A domains; it is present in the cartilage extracellular matrix and has a role in the development and homeostasis of cartilage and bone. Mutations in this gene result in multiple epiphyseal dysplasia.

NCBI and Uniprot Product Information

• NCBI GI #: 146218451
• NCBI GeneID: 4148
• UniProt Accession #: O15232
• NCBI Official Full Name: Matrilin 3
• NCBI Official Synonym Full Names: matrilin 3
• NCBI Official Symbol: MATN3
• NCBI Official Synonym Symbols: HOA; OS2; EDM5; DIPOA; OADIP
• NCBI Protein Information: matrilin-3
• UniProt Protein Name: Matrilin-3
• Protein Family: Matrilin
• UniProt Gene Name: MATN3
• UniProt Entry Name: MATN3_HUMAN

NCBI Description

This gene encodes a member of von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains two von Willebrand factor A domains; it is present in the cartilage extracellular matrix and has a role in the development and homeostasis of cartilage and bone. Mutations in this gene result in multiple epiphyseal dysplasia. [provided by RefSeq, Jul 2008]

Uniprot Description

MATN3: Major component of the extracellular matrix of cartilage and may play a role in the formation of extracellular filamentous networks. Defects in MATN3 are the cause of multiple epiphyseal dysplasia type 5 (EDM5). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. EDM5 is relatively mild and clinically variable. It is primarily characterized by delayed and irregular ossification of the epiphyses and early-onset osteoarthritis. Defects in MATN3 are the cause of spondyloepimetaphyseal dysplasia MATN3-related (SEMD-MATN3). A bone disease characterized by disproportionate early-onset dwarfism, bowing of the lower limbs, lumbar lordosis and normal hands. Skeletal abnormalities include short, wide and stocky long bones with severe epiphyseal and metaphyseal changes, hypoplastic iliac bones and flat, ovoid vertebral bodies. Genetic variations in MATN3 are associated with susceptibility to osteoarthritis type 2 (OS2); also called osteoarthritis of distal interphalangeal joints (OADIP) or hand osteoarthritis (HOA). Osteoarthritis is a degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement. In the hand, osteoarthritis can develop in the distal interphalangeal and the first carpometacarpal (base of thumb) and proximal interphalangeal joints. Patients with osteoarthritis may have one, a few, or all of these sites affected.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p24-p23

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: protein binding; extracellular matrix structural constituent

Biological Process: extracellular matrix organization and biogenesis; skeletal development

Disease: Osteoarthritis Susceptibility 2; Spondyloepimetaphyseal Dysplasia, Matrilin-3 Related; Epiphyseal Dysplasia, Multiple, 5