Rabbit anti-Human, Rat PLA1A Polyclonal Antibody | anti-PLA1A antibody

PLA1A Antibody

Cat. Num. AAA153483

• Gene Names: PLA1A; PSPLA1; PS-PLA1
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: PLA1A Antibody is affinity chromatography purified via peptide column.
• Synonyms: PLA1A; Polyclonal Antibody; PLA1A Antibody; PSPLA1; PS-PLA1; NMD; Phospholipase A1 member A; Phosphatidylserine-specific phospholipase A1; phospholipase A1 member A; anti-PLA1A antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least three isoforms of PLA1A are known to exist; this antibody will detect all three isoforms.
• Purity/Purification: PLA1A Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 456

• Applicable Applications for anti-PLA1A antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: PLA1A antibody can be used for detection of PLA1A by Western blot at 1 - 2 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: PLA1A antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human PLA1A.
• Buffer: PLA1A Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PLA1A antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PLA1A in human kidney tissue lysate with PLA1A antibody at (A) 1 and (B) 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of PLA1A in human kidney tissue with PLA1A antibody at 20 μg/mL.)

Related Product Information for anti-PLA1A antibody

PLA1A Antibody: PLA1A is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine (PS) in liposomes and can also hydrolyze PS in apoptotic cells and activate platelets where the resulting 2-acyl-lysophosphatidylserine acts as a lipid mediator for mast cells, T cells, and neural cells, suggesting that a major function of PLA1A may be the production of lysophospholipid mediators. PLA1A is upregulated in rat peripheral blood cells bearing long-term surviving cardiac allograft. PLA1A is also expressed in human THP-1-derived macrophages and this expression is upregulated in cells treated with lipopolysaccharide, a TLR4 ligand. This upregulation is inhibited with corticosteroids, which are often used at high dosages to suppress chronic allograft rejection.

NCBI and Uniprot Product Information

• NCBI GI #: 7706661
• NCBI GeneID: 51365
• NCBI Accession #: NP_056984
• NCBI GenBank Nucleotide #: NM_015900.3
• UniProt Accession #: Q53H76; O95991; Q86WX6; Q9UPD2; B2R8V2; B4DXA2
• Molecular Weight: 50 kDa
• NCBI Official Full Name: phospholipase A1 member A isoform 1
• NCBI Official Synonym Full Names: phospholipase A1 member A
• NCBI Official Symbol: PLA1A
• NCBI Official Synonym Symbols: PSPLA1; PS-PLA1
• NCBI Protein Information: phospholipase A1 member A; phosphatidylserine-specific phospholipase A1alpha
• UniProt Protein Name: Phospholipase A1 member A
• Protein Family: Phospholipase
• UniProt Gene Name: PLA1A
• UniProt Synonym Gene Names: NMD; PSPLA1; PS-PLA1
• UniProt Entry Name: PLA1A_HUMAN

NCBI Description

The protein encoded by this gene is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine in liposomes. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]

Uniprot Description

PLA1A: Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1- acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Isoform 2 hydrolyzes lyso-PS but not PS. Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production. Belongs to the AB hydrolase superfamily. Lipase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Phospholipase; Secreted, signal peptide; EC 3.1.1.-

Chromosomal Location of Human Ortholog: 3q13.13-q13.2

Cellular Component: acrosomal membrane

Molecular Function: phospholipase A1 activity

Biological Process: phosphatidylserine metabolic process; lipid metabolic process; lipid catabolic process