Human Mannan-binding lectin serine protease 2 ELISA Kit | MASP2 elisa kit

Human Mannan-binding lectin serine protease 2 ELISA Kit

Cat. Num. AAA764994

• Gene Names: MASP2; sMAP; MAP19; MASP-2; MASP1P1
• Reactivity: Human
• Synonyms: Mannan-binding lectin serine protease 2; Human Mannan-binding lectin serine protease 2 ELISA Kit; MASP2/MBL-associated serine protease 2/Mannose-binding protein-associated serine protease 2/MASP-2; MASP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MASP2 . No significant cross-reactivity or interference between MASP2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross - reactivity detection between MASP2 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 686

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 3.125-200ng/ml
• Sensitivity: < 1.875ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MASP2were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MASP2were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MASP2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MASP2antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MASP2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MASP2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MASP2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 21264363
• NCBI GeneID: 10747
• NCBI Accession #: NP_006601.2
• NCBI GenBank Nucleotide #: NM_006610.3
• Molecular Weight: 20,629 Da
• NCBI Official Full Name: mannan-binding lectin serine protease 2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: mannan-binding lectin serine peptidase 2
• NCBI Official Symbol: MASP2
• NCBI Official Synonym Symbols: sMAP; MAP19; MASP-2; MASP1P1
• NCBI Protein Information: mannan-binding lectin serine protease 2
• UniProt Protein Name: Mannan-binding lectin serine protease 2
• Protein Family: Mannan-binding lectin serine protease
• UniProt Gene Name: MASP2
• UniProt Synonym Gene Names: MASP-2
• UniProt Entry Name: MASP2_HUMAN

NCBI Description

The Ra-reactive factor (RARF) is a complement-dependent bactericidal factor that binds to the Ra and R2 polysaccharides expressed by certain enterobacteria. Alternate splicing of this gene results in two transcript variants encoding two RARF components that are involved in the mannan-binding lectin pathway of complement activation. The longer isoform is cleaved into two chains which form a heterodimer linked by a disulfide bond. The encoded proteins are members of the trypsin family of peptidases. [provided by RefSeq, Jul 2008]

Uniprot Description

MASP2: Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase. Defects in MASP2 are the cause of MASP2 deficiency (MASPD). MASPD is a disorder that results in autoimmune manifestations, recurrent severe infections, and chronic inflammatory disease. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; EC 3.4.21.104; Protease

Chromosomal Location of Human Ortholog: 1p36.3-p36.2

Cellular Component: extracellular region

Molecular Function: calcium ion binding; calcium-dependent protein binding; complement component C4b binding; peptidase activity; protein binding; serine-type endopeptidase activity

Biological Process: complement activation; complement activation, classical pathway; complement activation, lectin pathway; innate immune response; proteolysis

Disease: Masp2 Deficiency