JNK1 recombinant protein

JNK1, Active

Cat. Num. AAA515544

• Gene Names: Mapk8; JNK; JNK1; Prkm8; SAPK1; AI849689
• Applications: Western Blot
• Synonyms: JNK1; Active; JNK1 recombinant protein

• Host: Sf9 insect cells using baculovirus
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Sequence Positions: Full Length
• Sequence Length: 5770

• Applicable Applications for JNK1 recombinant protein: Kinase Assay, Western Blot (WB)
• Species: Mouse
• Tag: GST tag
• Preparation and Storage: Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Testing Data #2

Related Product Information for JNK1 recombinant protein

Description: Recombinant full-length mouse JNK1 was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Scientific Background: JNK1 is a member of the MAP kinase group that is activated by dual phosphorylation at thr and tyr residues during exposure to stress such as UV irradiation. JNK1 binds to the c-Jun transactivation domain and phosphorylates it on Ser-63 and Ser-73 (1). JNK1 has been shown to play an important role in disease processes. Activation of JNK1 results in defects in myotube viability and integrity leading to dystrophic myofiber destruction (2). JNK1 activity is also abnormally elevated in obesity and removal of JNK1 results in decreased adiposity and significantly improved insulin sensitivity.

Product Categories/Family for JNK1 recombinant protein

Enzymes; Active Kinases

References

1. Derijard, B. et al: JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Cell. 1994 Mar 25;76 (6):1025-37. 2. Kolodziejczyk, S M. et al: Activation of JNK1 contributes to dystrophic muscle pathogenesis. Curr Biol. 2001 Aug 21;11 (16):1278-82. 3. Liu. B, et al. ABL-N-induced apoptosis in human breast cancer cells is partially mediated by c-Jun NH2-terminal kinase activation. Breast Cancer Res. 2010, 12 (1):R9

NCBI and Uniprot Product Information

• NCBI GI #: 225637490
• NCBI GeneID: 26419
• NCBI Accession #: NM_016700.4
• NCBI GenBank Nucleotide #: NM_016700
• UniProt Accession #: Q91Y86
• Molecular Weight: ~71 kDa
• NCBI Official Full Name: Mus musculus mitogen-activated protein kinase 8 (Mapk8), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8
• NCBI Official Symbol: Mapk8
• NCBI Official Synonym Symbols: JNK; JNK1; Prkm8; SAPK1; AI849689
• NCBI Protein Information: mitogen-activated protein kinase 8
• UniProt Protein Name: Mitogen-activated protein kinase 8
• Protein Family: JNK1/MAPK8-associated membrane protein
• UniProt Gene Name: Mapk8
• UniProt Synonym Gene Names: Jnk1; Prkm8; MAP kinase 8; MAPK 8

Uniprot Description

JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described.

Protein type: CMGC group; EC 2.7.11.24; JNK subfamily; Kinase, protein; MAPK family; MAPK/JNK subfamily; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 14|14 B

Cellular Component: axon; cytoplasm; cytosol; dendrite cytoplasm; mitochondrion; neuron projection; nucleus; perikaryon; podosome; vesicle

Molecular Function: enzyme binding; histone deacetylase binding; histone deacetylase regulator activity; JUN kinase activity; kinase activity; kinesin binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: cellular response to amino acid starvation; dendrite morphogenesis; determination of dorsal identity; inflammatory response; JNK cascade; JUN phosphorylation; negative regulation of apoptosis; negative regulation of protein binding; neurite development; neuron migration; ossification; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of cell migration; positive regulation of cyclase activity; positive regulation of DNA replication; positive regulation of microtubule polymerization; positive regulation of neuroblast proliferation; positive regulation of neuron apoptosis; positive regulation of protein metabolic process; protein amino acid phosphorylation; regulation of centrosome cycle; regulation of circadian rhythm; regulation of gene expression; regulation of neuron differentiation; regulation of protein localization; regulation of transcription, DNA-dependent; response to cadmium ion; response to heat; response to hydrogen peroxide; response to mechanical stimulus; response to osmotic stress; response to UV; signal transduction; stress-activated MAPK cascade