Dual specificity mitogen-activated protein kinase kinase 4 Recombinant Protein | MP2K4 recombinant protein

Recombinant mouse Dual specificity mitogen-activated protein kinase kinase 4

Cat. Num. AAA9420438

• Gene Names: Map2k4; MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4
• Reactivity: Mouse
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Dual specificity mitogen-activated protein kinase kinase 4; Recombinant mouse Dual specificity mitogen-activated protein kinase kinase 4; C-JUN N-terminal kinase kinase 1; JNK kinase 1; JNKK 1JNK-activating kinase 1MAPK/ERK kinase 4; MEK 4SAPK/ERK kinase 1; SEK1; MP2K4 recombinant protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Full Length, 2-397a

• Tag Info: His-SUMO-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for MP2K4 recombinant protein

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 ses to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.

NCBI and Uniprot Product Information

• NCBI GI #: 22095023
• NCBI GeneID: 26398
• NCBI Accession #: NP_033183.1
• NCBI GenBank Nucleotide #: NM_009157.5
• UniProt Accession #: P47809
• Molecular Weight: 60kD
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 4 isoform b
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 4
• NCBI Official Symbol: Map2k4
• NCBI Official Synonym Symbols: MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 4
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 4
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: Map2k4
• UniProt Synonym Gene Names: Jnkk1; Mek4; Mkk4; Prkmk4; Sek1; Serk1; Skk1; MAP kinase kinase 4; MAPKK 4; JNK kinase 1; JNKK 1; MEK 4; SEK1

Uniprot Description

MKK4: dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38 but not ERK1 or -2. Mediates cellular responses to various cellular stresses and inflammatory cytokines. Phosphorylation by Akt inhibits MKK4 and suppresses stress-activated signal transduction.

Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 11 B3|11 40.53 cM

Cellular Component: axon; cytoplasm; cytosol; dendrite cytoplasm; nucleus; perikaryon

Molecular Function: ATP binding; JUN kinase kinase activity; MAP kinase kinase activity; mitogen-activated protein kinase kinase kinase binding; protein binding; protein serine/threonine kinase activity

Biological Process: activation of JNK activity; activation of protein kinase activity; JNK cascade; MAPKKK cascade; positive regulation of apoptosis; positive regulation of DNA replication; positive regulation of neuron apoptosis; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; regulation of mitotic cell cycle; response to wounding; stress-activated protein kinase signaling pathway