5'-Nucleotidase (Nt5e) Active Protein | Nt5e active protein

Recombinant Mouse 5'-Nucleotidase (Nt5e), Partial

Cat. Num. AAA7115107

• Gene Names: Nt5e; NT; Nt5; eNT; CD73; 5'-NT; AI447961; 2210401F01Rik
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: 5'-Nucleotidase (Nt5e); Recombinant Mouse 5'-Nucleotidase (Nt5e); Partial; 5'-nucleotidase; Ecto-5'-nucleotidase; CD73; 5'-NT; Nt5e active protein

• Host: Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: 0.2 mum Filtered 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% Glycerol, pH 7.5
• Sequence Positions: 29-550aa; Partial
• Sequence: WELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLFLDAGDQYQGTIWFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISGLFLPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVVQAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRIKF
• Sequence Length: 576

• Species: Mouse
• Tag: C-terminal 6xHis-tagged
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Biological Activity: Specific activity as determined by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP) is greater than 10000 pmol/min/ug. The orthophosphate product is measured by a malachite green phosphate detection kit.
• Subcellular Location: Cell Membrane, Lipid-Anchor, GPI-Anchor
• Protein Families: 5'-Nucleotidase Family
• Classification: Drug Target
• Subdivision: CD Antigen
• Preparation and Storage: Store at -20 degree C. For extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for Nt5e active protein

Relevance: Mouse CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.

Function: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Product Categories/Family for Nt5e active protein

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 13431280
• NCBI GeneID: 23959
• UniProt Accession #: Q61503
• Molecular Weight: 58.8 kDa
• NCBI Official Full Name: 5'-nucleotidase
• NCBI Official Synonym Full Names: 5' nucleotidase, ecto
• NCBI Official Symbol: Nt5e
• NCBI Official Synonym Symbols: NT; Nt5; eNT; CD73; 5'-NT; AI447961; 2210401F01Rik
• NCBI Protein Information: 5'-nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• UniProt Gene Name: Nt5e
• UniProt Synonym Gene Names: Nt5; Nte; 5'-NT

NCBI Description

This gene encodes a membrane-bound nucleotidase that hydrolyzes extracellular nucleoside monophosphates. The encoded preproprotein undergoes proteolytic processing to generate to a functional, homodimeric enzyme that preferentially uses adenosine monophosphate as a substrate to generate free adenosine. Mice lacking the encoded protein exhibit a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow. [provided by RefSeq, Sep 2016]

Uniprot Description

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.